BMRB Entry 17939

Name: a protein from Haloferax volcanii
Published: 2012-10-09

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PDB ID: 2lji
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17939
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

a protein from Haloferax volcanii

Deposition date:

2011-09-14

Original release date:

2012-10-09

Authors:

Zhang, Wen; Liao, Shanhui; Fan, Kai

Citation:

Citation: Liao, Shanhui; Zhang, Wen; Fan, Kai; Tu, Xiaoming. "structure of a protein from Haloferax volcanii" .

Assembly members:

Assembly members:
a protein from Haloferax volcanii, polymer, 132 residues, 14231.962 Da.

Natural source:

Natural source: Common Name: Haloferax volcanii Taxonomy ID: 2246 Superkingdom: Archaea Kingdom: not available Genus/species: Haloferax volcanii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
a protein from Haloferax volcanii: MNVTVEVVGEETSEVAVDDD GTYADLVRAVDLSPHEVTVL VDGRPVPEDQSVEVDRVKVL RLIKGGMNVTVEVVGEETSE VAVDDDGTYADLVRAVDLSP HEVTVLVDGRPVPEDQSVEV DRVKVLRLIKGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	98
15N chemical shifts	55
1H chemical shifts	339

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	a protein from Haloferax volcanii	1

Entities:

Entity 1, a protein from Haloferax volcanii 132 residues - 14231.962 Da.

1

MET

ASN

VAL

THR

VAL

GLU

VAL

GLY

GLU

2

GLU

THR

SER

GLU

VAL

ALA

VAL

ASP

3

GLY

THR

TYR

ALA

ASP

LEU

VAL

ARG

ALA

VAL

4

ASP

LEU

SER

PRO

HIS

GLU

VAL

THR

VAL

LEU

5

VAL

ASP

GLY

ARG

PRO

VAL

PRO

GLU

ASP

GLN

6

SER

VAL

GLU

VAL

ASP

ARG

VAL

LYS

VAL

LEU

7

ARG

LEU

ILE

LYS

GLY

MET

ASN

VAL

THR

8

VAL

GLU

VAL

GLY

GLU

THR

SER

GLU

9

VAL

ALA

VAL

ASP

GLY

THR

TYR

ALA

10

ASP

LEU

VAL

ARG

ALA

VAL

ASP

LEU

SER

PRO

11

HIS

GLU

VAL

THR

VAL

LEU

VAL

ASP

GLY

ARG

12

PRO

VAL

PRO

GLU

ASP

GLN

SER

VAL

GLU

VAL

13

ASP

ARG

VAL

LYS

VAL

LEU

ARG

LEU

ILE

LYS

14

GLY

Samples:

sample_1: a protein from Haloferax volcanii, [15N;13C], 0.6 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_2: a protein from Haloferax volcanii, [15N;13C], 0.6 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O 100%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift calculation

NMR spectrometers:

Bruker DMX 500 MHz

BMRB	17204
PDB	2L32 2LJI
GB	ADE03392 AKU06380 ELK50451 ELY23894 ELZ70132
REF	WP_004045316 WP_004065555 WP_008609462
SP	D4GZE7
AlphaFold	D4GZE7