BMRB Entry 17935

Click here to enlarge.

PDB ID: 2lxn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17935
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C, 15N assignment and secondary structure determination of GATase subunit of GMP Synthetase from Methanococcus jannaschii
Deposition date:
2011-09-13
Original release date:
2011-12-20
Authors:
Ali, Rustam; Sarma, Siddhartha
Citation:

Citation: Ali, Rustam; Kumar, Sanjeev; Balaram, Hemalatha; Sarma, Siddhartha. "1H, 13C, 15N assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii."  Biomol. NMR Assignments 6, 193-196 (2012).
PubMed: 22203461

Assembly members:

Assembly members:
GATase_subunit, polymer, 188 residues, 21020.2 Da.

Natural source:

Natural source:   Common Name: Methanococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3atr

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GATase_subunit: MIVILDNGGQYVHRIHRSLK YIGVSSKIVPNTTPLEEIES NKEVKGIILSGGPDIEKAKN CIDIALNAKLPILGICLGHQ LIALAYGGEVGRAEAEEYAL TKVYVDKENDLFKNVPREFN AWASHKDEVKKVPEGFEILA HSDICQVEAMKHKTKPIYGV QFHPEVAHTEYGNEILKNFC KVCGYKFE

Data sets:
Data typeCount
13C chemical shifts395
15N chemical shifts185
1H chemical shifts857

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GATase subunit1

Entities:

Entity 1, GATase subunit 188 residues - 21020.2 Da.

1   METILEVALILELEUASPASNGLYGLYGLN
2   TYRVALHISARGILEHISARGSERLEULYS
3   TYRILEGLYVALSERSERLYSILEVALPRO
4   ASNTHRTHRPROLEUGLUGLUILEGLUSER
5   ASNLYSGLUVALLYSGLYILEILELEUSER
6   GLYGLYPROASPILEGLULYSALALYSASN
7   CYSILEASPILEALALEUASNALALYSLEU
8   PROILELEUGLYILECYSLEUGLYHISGLN
9   LEUILEALALEUALATYRGLYGLYGLUVAL
10   GLYARGALAGLUALAGLUGLUTYRALALEU
11   THRLYSVALTYRVALASPLYSGLUASNASP
12   LEUPHELYSASNVALPROARGGLUPHEASN
13   ALATRPALASERHISLYSASPGLUVALLYS
14   LYSVALPROGLUGLYPHEGLUILELEUALA
15   HISSERASPILECYSGLNVALGLUALAMET
16   LYSHISLYSTHRLYSPROILETYRGLYVAL
17   GLNPHEHISPROGLUVALALAHISTHRGLU
18   TYRGLYASNGLUILELEULYSASNPHECYS
19   LYSVALCYSGLYTYRLYSPHEGLU

Related Database Links:

PDB 2LXN
GB AAB99597
SP Q58970
AlphaFold Q58970

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks