BMRB Entry 17883
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PDB ID: 2li8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17883
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Loughlin, Fionna; Gebert, Luca; Towbin, Harry; Brunschweiger, Andreas; Hall, Jonathan; Allain, Frederic H-T. "Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28." Nat. Struct. Mol. Biol. 19, 84-89 (2012).
PubMed: 22157959
Assembly members:
Lin28-ZnF, polymer, 74 residues, 8033.417 Da.
AGGAGAU, polymer, 7 residues, 2284.459 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Lin28-ZnF: SSGLVPRGSHMPKGKSMQKR
RSKGDRCYNCGGLDHHAKEC
KLPPQPKKCHFCQSISHMVA
SCPLKAQQGPSAQG
AGGAGAU: AGGAGAU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 191 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 450 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lin-28 homolog A | 1 |
| 2 | hsa-pre-let-7g miRNA | 2 |
| 3 | ZINC ION | 3 |
Entities:
Entity 1, Lin-28 homolog A 74 residues - 8033.417 Da.
Residues 1-11 a non native expression tag
| 1 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 2 | MET | PRO | LYS | GLY | LYS | SER | MET | GLN | LYS | ARG | ||||
| 3 | ARG | SER | LYS | GLY | ASP | ARG | CYS | TYR | ASN | CYS | ||||
| 4 | GLY | GLY | LEU | ASP | HIS | HIS | ALA | LYS | GLU | CYS | ||||
| 5 | LYS | LEU | PRO | PRO | GLN | PRO | LYS | LYS | CYS | HIS | ||||
| 6 | PHE | CYS | GLN | SER | ILE | SER | HIS | MET | VAL | ALA | ||||
| 7 | SER | CYS | PRO | LEU | LYS | ALA | GLN | GLN | GLY | PRO | ||||
| 8 | SER | ALA | GLN | GLY |
Entity 2, hsa-pre-let-7g miRNA 7 residues - 2284.459 Da.
| 1 | A | G | G | A | G | A | U |
Entity 3, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Related Database Links:
| PDB | 2CQF 2LI8 |
| DBJ | BAB14075 BAI46599 |
| GB | AAH28566 AAM77751 ABM84324 ABM87718 AIC52303 |
| REF | NP_001102739 NP_078950 XP_001114640 XP_002716148 XP_002720890 |
| SP | Q9H9Z2 |
| AlphaFold | Q9H9Z2 |
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SPARKY: Backbone
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