BMRB Entry 17867
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PDB ID: 2lhu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17867
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Howarth, Jack; Ramisetti, Srinivas; Nolan, Kristof; Sadayappan, Sakthivel; Rosevear, Paul. "Structural Insight into Unique Cardiac Myosin-binding Protein-C Motif: A PARTIALLY FOLDED DOMAIN." J. Biol. Chem. 287, 8254-8262 (2012).
PubMed: 22235120
Assembly members:
Myosin Binding Protein-C Motif, polymer, 124 residues, 4189.856 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Myosin Binding Protein-C Motif: MGSSHHHHHHSSGLVPRGSH
MHEAIGSGDLDLRSAFRRTS
LAGAGRRTSDSHEDAGTLDF
SSLLKKRDSFRRDSKLEAPA
EEDVWEILRQAPPSEYERIA
FQHGVTDLRGMLKRLKGMKQ
DEKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 298 |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 210 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Myosin Binding Protein-C Motif | 1 |
Entities:
Entity 1, Myosin Binding Protein-C Motif 124 residues - 4189.856 Da.
Residues 1-20 represent a histidine tag.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | HIS | GLU | ALA | ILE | GLY | SER | GLY | ASP | LEU | ||||
| 4 | ASP | LEU | ARG | SER | ALA | PHE | ARG | ARG | THR | SER | ||||
| 5 | LEU | ALA | GLY | ALA | GLY | ARG | ARG | THR | SER | ASP | ||||
| 6 | SER | HIS | GLU | ASP | ALA | GLY | THR | LEU | ASP | PHE | ||||
| 7 | SER | SER | LEU | LEU | LYS | LYS | ARG | ASP | SER | PHE | ||||
| 8 | ARG | ARG | ASP | SER | LYS | LEU | GLU | ALA | PRO | ALA | ||||
| 9 | GLU | GLU | ASP | VAL | TRP | GLU | ILE | LEU | ARG | GLN | ||||
| 10 | ALA | PRO | PRO | SER | GLU | TYR | GLU | ARG | ILE | ALA | ||||
| 11 | PHE | GLN | HIS | GLY | VAL | THR | ASP | LEU | ARG | GLY | ||||
| 12 | MET | LEU | LYS | ARG | LEU | LYS | GLY | MET | LYS | GLN | ||||
| 13 | ASP | GLU | LYS | LYS |
Related Database Links:
| PDB | 2LHU |
| DBJ | BAE27506 BAE41061 |
| GB | AAC64202 AAI54409 EDL27527 |
| PRF | 2020397A |
| REF | NP_032679 XP_006234565 XP_006234566 XP_006498945 XP_011237643 |
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