BMRB Entry 17833

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PDB ID: 2n4i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17833
MolProbity Validation Chart

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Title:
Skint1 IgV
Deposition date:
2011-08-05
Original release date:
2016-02-29
Authors:
Salim, Mahboob; Knowles, Timothy; Willcox, Carrie; Mohammed, Fiyaz; Woodard, Martin; Overduin, Michael; Hayday, Adrian; Willcox, Benjamin
Citation:

Citation: Salim, Mahboob; Knowles, Timothy; Willcox, Carrie; Mohammed, Fiyaz; Woodard, Martin; Overduin, Michael; Hayday, Adrian; Willcox, Benjamin. "Characterisation of a putative receptor binding surface on Skint-1, a critical determinant of dendritic epidermal T cell selection"  J. Biol. Chem. ., .-. (2016).

Assembly members:

Assembly members:
Skint1_IgV, polymer, 238 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Skint1_IgV: MSSEPFIVNGLEGPVLASLG GNLELSCQLSPPQQAQHMEI RWFRNLYTEPVHLYRDGKDM FGEIISKYVERTELLKDGIG EGKVTLRIFNVTVDDDGSYH CVFKDGDFYEEHITEVKITM SSEPFIVNGLEGPVLASLGG NLELSCQLSPPQQAQHMEIR WFRNLYTEPVHLYRDGKDMF GEIISKYVERTELLKDGIGE GKVTLRIFNVTVDDDGSYHC VFKDGDFYEEHITEVKIT

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts99
1H chemical shifts641

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Skint1 IgV1

Entities:

Entity 1, Skint1 IgV 238 residues - Formula weight is not available

2 to 119 is the distal membrane Ig variable domain

1   METSERSERGLUPROPHEILEVALASNGLY
2   LEUGLUGLYPROVALLEUALASERLEUGLY
3   GLYASNLEUGLULEUSERCYSGLNLEUSER
4   PROPROGLNGLNALAGLNHISMETGLUILE
5   ARGTRPPHEARGASNLEUTYRTHRGLUPRO
6   VALHISLEUTYRARGASPGLYLYSASPMET
7   PHEGLYGLUILEILESERLYSTYRVALGLU
8   ARGTHRGLULEULEULYSASPGLYILEGLY
9   GLUGLYLYSVALTHRLEUARGILEPHEASN
10   VALTHRVALASPASPASPGLYSERTYRHIS
11   CYSVALPHELYSASPGLYASPPHETYRGLU
12   GLUHISILETHRGLUVALLYSILETHRMET
13   SERSERGLUPROPHEILEVALASNGLYLEU
14   GLUGLYPROVALLEUALASERLEUGLYGLY
15   ASNLEUGLULEUSERCYSGLNLEUSERPRO
16   PROGLNGLNALAGLNHISMETGLUILEARG
17   TRPPHEARGASNLEUTYRTHRGLUPROVAL
18   HISLEUTYRARGASPGLYLYSASPMETPHE
19   GLYGLUILEILESERLYSTYRVALGLUARG
20   THRGLULEULEULYSASPGLYILEGLYGLU
21   GLYLYSVALTHRLEUARGILEPHEASNVAL
22   THRVALASPASPASPGLYSERTYRHISCYS
23   VALPHELYSASPGLYASPPHETYRGLUGLU
24   HISILETHRGLUVALLYSILETHR

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