BMRB Entry 17825
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PDB ID: 2lgw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17825
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gao, Xue-Chao; Zhou, Chen-Jie; Zhou, Zi-Ren; Wu, Meng; Cao, Chun-Yang; Hu, Hong-Yu. "The C-terminal helices of heat shock protein 70 are essential for J-domain binding and ATPase activation." J. Biol. Chem. 287, 6044-6052 (2012).
PubMed: 22219199
Assembly members:
entity, polymer, 99 residues, 8556.679 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b
Entity Sequences (FASTA):
entity: MASYYEILDVPRSASADDIK
KAYRRKALQWHPDKNPDNKE
FAEKKFKEVAEAYEVLSDKH
KREIYDRYGREPLTGTGTGP
SRAEAGSGGPGLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 185 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 367 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HSJ1a | 1 |
Entities:
Entity 1, HSJ1a 99 residues - 8556.679 Da.
| 1 | MET | ALA | SER | TYR | TYR | GLU | ILE | LEU | ASP | VAL | ||||
| 2 | PRO | ARG | SER | ALA | SER | ALA | ASP | ASP | ILE | LYS | ||||
| 3 | LYS | ALA | TYR | ARG | ARG | LYS | ALA | LEU | GLN | TRP | ||||
| 4 | HIS | PRO | ASP | LYS | ASN | PRO | ASP | ASN | LYS | GLU | ||||
| 5 | PHE | ALA | GLU | LYS | LYS | PHE | LYS | GLU | VAL | ALA | ||||
| 6 | GLU | ALA | TYR | GLU | VAL | LEU | SER | ASP | LYS | HIS | ||||
| 7 | LYS | ARG | GLU | ILE | TYR | ASP | ARG | TYR | GLY | ARG | ||||
| 8 | GLU | PRO | LEU | THR | GLY | THR | GLY | THR | GLY | PRO | ||||
| 9 | SER | ARG | ALA | GLU | ALA | GLY | SER | GLY | GLY | PRO | ||||
| 10 | GLY | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Related Database Links:
| PDB | 2LGW |
| DBJ | BAE87866 BAE88406 BAF82315 BAF85450 BAG35597 |
| EMBL | CAA44968 CAA44969 |
| GB | AAA09034 AAA09035 AAH11609 AAH47056 AAP35751 |
| REF | NP_001034639 NP_001162567 NP_001253510 NP_001267342 NP_001271020 |
| SP | P25686 |
| AlphaFold | P25686 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks