BMRB Entry 17820

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17820

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Title:
Backbone resonance assignments for IsdH-N2N3
Deposition date:
2011-07-29
Original release date:
2011-12-12
Authors:
Spirig, Thomas; Clubb, Robert
Citation:

Citation: Spirig, Thomas; Clubb, Robert. "Backbone 1H, 13C and 15N resonance assignments of the 39 kDa staphylococcal hemoglobin receptor IsdH."  Biomol. NMR Assignments 6, 169-172 (2012).
PubMed: 22101872

Assembly members:

Assembly members:
IsdH-N2N3, polymer, 336 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: -

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IsdH-N2N3: SADESLQDAIKNPAIIDKEH TADNWRPIDFQMKNDKGERQ FYHYASTVEPATVIFTKTGP IIELGLKTASTWKKFEVYEG DKKLPVELVSYDSDKDYAYI RFPVSNGTREVKIVSSIEYG ENIHEDYDYTLMVFAQPITN NPDDYVDEETYNLQKLLAPY HKAKTLERQVYELEKLQEKL PEKYKAEYKKKLDQTRVELA DQVKSAVTEFENVTPTNDQL TDLQEAHFVVFESEENSESV MDGFVEHPFYTATLNGQKYV VMKTKDDSYWKDLIVEGKRV TTVSKDPKNNSRTLIFPYIP DKAVYNAIVKVVVANIGAEG QYHVRIINQDINTKDD

Data sets:
Data typeCount
13C chemical shifts879
15N chemical shifts292
1H chemical shifts292

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IsdH-N2N31

Entities:

Entity 1, IsdH-N2N3 336 residues - Formula weight is not available

Residues 1 represents a non-native residue remaining after cleavage of a purification tag.

1   SERALAASPGLUSERLEUGLNASPALAILE
2   LYSASNPROALAILEILEASPLYSGLUHIS
3   THRALAASPASNTRPARGPROILEASPPHE
4   GLNMETLYSASNASPLYSGLYGLUARGGLN
5   PHETYRHISTYRALASERTHRVALGLUPRO
6   ALATHRVALILEPHETHRLYSTHRGLYPRO
7   ILEILEGLULEUGLYLEULYSTHRALASER
8   THRTRPLYSLYSPHEGLUVALTYRGLUGLY
9   ASPLYSLYSLEUPROVALGLULEUVALSER
10   TYRASPSERASPLYSASPTYRALATYRILE
11   ARGPHEPROVALSERASNGLYTHRARGGLU
12   VALLYSILEVALSERSERILEGLUTYRGLY
13   GLUASNILEHISGLUASPTYRASPTYRTHR
14   LEUMETVALPHEALAGLNPROILETHRASN
15   ASNPROASPASPTYRVALASPGLUGLUTHR
16   TYRASNLEUGLNLYSLEULEUALAPROTYR
17   HISLYSALALYSTHRLEUGLUARGGLNVAL
18   TYRGLULEUGLULYSLEUGLNGLULYSLEU
19   PROGLULYSTYRLYSALAGLUTYRLYSLYS
20   LYSLEUASPGLNTHRARGVALGLULEUALA
21   ASPGLNVALLYSSERALAVALTHRGLUPHE
22   GLUASNVALTHRPROTHRASNASPGLNLEU
23   THRASPLEUGLNGLUALAHISPHEVALVAL
24   PHEGLUSERGLUGLUASNSERGLUSERVAL
25   METASPGLYPHEVALGLUHISPROPHETYR
26   THRALATHRLEUASNGLYGLNLYSTYRVAL
27   VALMETLYSTHRLYSASPASPSERTYRTRP
28   LYSASPLEUILEVALGLUGLYLYSARGVAL
29   THRTHRVALSERLYSASPPROLYSASNASN
30   SERARGTHRLEUILEPHEPROTYRILEPRO
31   ASPLYSALAVALTYRASNALAILEVALLYS
32   VALVALVALALAASNILEGLYALAGLUGLY
33   GLNTYRHISVALARGILEILEASNGLNASP
34   ILEASNTHRLYSASPASP

Samples:

sample_1: IsdH-N2N3, [U-100% 13C; U-100% 15N; U-80% 2H], 1.1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.01%; D2O, [U-2H], 7%; H2O 93%; Protease Inhibitor Cocktail 0.01%

sample_conditions_1: ionic strength: 70 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CAI81279 COX93366 CPE78318 CPJ07879 CPL95539
GB AIW27424 EEV26426 EFB57600 EFB60931 EFC00882
REF WP_042745476 WP_052997956 WP_053014930

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks