BMRB Entry 17816
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17816
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Turner, David; Lamosa, Pedro; Martinez, Beatriz. "Structure and properties of lactococcin 972 from Lactococcus lactis" .
Assembly members:
Lcn972, polymer, 66 residues, 7392.028 Da.
Natural source: Common Name: Lactococcus lactis Taxonomy ID: 1358 Superkingdom: Bacteria Kingdom: not available Genus/species: Lactococcus lactis
Experimental source: Production method: purified from the natural source Host organism: Lactococcus lactis
Entity Sequences (FASTA):
Lcn972: EGTWQHGYGVSSAYSNYHHG
SKTHSATVVNNNTGRQGKDT
QRAGVWAKATVGRNLTEKAS
FYYNFW
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 423 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lcn972 | 1 |
Entities:
Entity 1, Lcn972 66 residues - 7392.028 Da.
| 1 | GLU | GLY | THR | TRP | GLN | HIS | GLY | TYR | GLY | VAL | ||||
| 2 | SER | SER | ALA | TYR | SER | ASN | TYR | HIS | HIS | GLY | ||||
| 3 | SER | LYS | THR | HIS | SER | ALA | THR | VAL | VAL | ASN | ||||
| 4 | ASN | ASN | THR | GLY | ARG | GLN | GLY | LYS | ASP | THR | ||||
| 5 | GLN | ARG | ALA | GLY | VAL | TRP | ALA | LYS | ALA | THR | ||||
| 6 | VAL | GLY | ARG | ASN | LEU | THR | GLU | LYS | ALA | SER | ||||
| 7 | PHE | TYR | TYR | ASN | PHE | TRP |
Samples:
sample_1: Lcn972 1.3 ± 0.1 mM; H2O 90 ± 0.1 %; D2O 10 ± 0.1 %
sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment, peak picking
TOPSPIN v3.0, Bruker Biospin - collection, processing
DYANA vINDYANA extended, Guntert, Braun and Wuthrich - refinement, structure solution
NMR spectrometers:
- Bruker Avance 800 MHz