BMRB Entry 17784

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PDB ID: 2lfw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17784
MolProbity Validation Chart

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Title:
NMR structure of the PhyRSL-NepR complex from Sphingomonas sp. Fr1
Deposition date:
2011-07-18
Original release date:
2012-04-18
Authors:
Campagne, Sebastien; Damberger, Fred; Vorholt, Julia; Allain, Frederic
Citation:

Citation: Campagne, Sebastien; Damberger, Fred; Kaczmarczyk, Andreas; Francez-Charlot, Anne; Allain, Frederic H-T; Vorholt, Julia. "Structural basis for sigma factor mimicry in the general stress response of Alphaproteobacteria."  Proc. Natl. Acad. Sci. U.S.A. 109, E1405-E1414 (2012).
PubMed: 22550171

Assembly members:

Assembly members:
PhyR_sigma_like_domain, polymer, 157 residues, 17336.539 Da.
NepR_anti_sigma_factor, polymer, 62 residues, 6923.098 Da.

Natural source:

Natural source:   Common Name: Sphingomonas sp. Fr1   Taxonomy ID: 907061   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Sphingomonas Sphingomonas sp. Fr1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET26bII-PhyRSL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PhyR_sigma_like_domain: MSLGQQLAPHLPFLRRYGRA LTGSQNQGDKYVRATLEAIV AAPDQFPRDVDPRLGLYRMF QGIWASANADGEAQTSQSDA EGTEAVARARLARMTPLSRQ ALLLTAMEGFSPEDAAYLIE VDTSEVETLVTEALAEIEKQ TRALELVPRGSHHHHHH
NepR_anti_sigma_factor: MLDLPGNKDKKASSKKSPAK VQSKDRDMGAALRSAYQKTI EEQVPDEMLDLLNKLALELV PR

Data sets:
Data typeCount
13C chemical shifts831
15N chemical shifts212
1H chemical shifts1415

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PhyR_sigma_like_domain1
2NepR_anti_sigma_factor2

Entities:

Entity 1, PhyR_sigma_like_domain 157 residues - 17336.539 Da.

From residue 144 to 157, amino acid residues of thrombin cleavage site + his tag

1   METSERLEUGLYGLNGLNLEUALAPROHIS
2   LEUPROPHELEUARGARGTYRGLYARGALA
3   LEUTHRGLYSERGLNASNGLNGLYASPLYS
4   TYRVALARGALATHRLEUGLUALAILEVAL
5   ALAALAPROASPGLNPHEPROARGASPVAL
6   ASPPROARGLEUGLYLEUTYRARGMETPHE
7   GLNGLYILETRPALASERALAASNALAASP
8   GLYGLUALAGLNTHRSERGLNSERASPALA
9   GLUGLYTHRGLUALAVALALAARGALAARG
10   LEUALAARGMETTHRPROLEUSERARGGLN
11   ALALEULEULEUTHRALAMETGLUGLYPHE
12   SERPROGLUASPALAALATYRLEUILEGLU
13   VALASPTHRSERGLUVALGLUTHRLEUVAL
14   THRGLUALALEUALAGLUILEGLULYSGLN
15   THRARGALALEUGLULEUVALPROARGGLY
16   SERHISHISHISHISHISHIS

Entity 2, NepR_anti_sigma_factor 62 residues - 6923.098 Da.

1   METLEUASPLEUPROGLYASNLYSASPLYS
2   LYSALASERSERLYSLYSSERPROALALYS
3   VALGLNSERLYSASPARGASPMETGLYALA
4   ALALEUARGSERALATYRGLNLYSTHRILE
5   GLUGLUGLNVALPROASPGLUMETLEUASP
6   LEULEUASNLYSLEUALALEUGLULEUVAL
7   PROARG

Related Database Links:

PDB 2LFW
GB AET62591 KIU26946 AET62590
REF WP_017979560 WP_043060725

Download HSQC peak lists in one of the following formats:
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