BMRB Entry 17780

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PDB ID: 2lft
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17780
MolProbity Validation Chart

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Title:
Human prion protein with E219K protective polymorphism
Deposition date:
2011-07-14
Original release date:
2012-06-25
Authors:
Biljan, Ivana; Ilc, Gregor; Giancin, Gabriele; Zhukov, Igor; Plavec, Janez; Legname, Guiseppe
Citation:

Citation: Biljan, Ivana; Giachin, Gabriele; Ilc, Gregor; Zhukov, Igor; Plavec, Janez; Legname, Giuseppe. "Structural basis for the protective effect of the human prion protein carrying the dominant-negative E219K polymorphism."  Biochem. J. 446, 243-251 (2012).
PubMed: 22676969

Assembly members:

Assembly members:
Hpp_E219K, polymer, 142 residues, 16169.169 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pProExHTa

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hpp_E219K: GQGGGTHSQWNKPSKPKTNM KHMAGAAAAGAVVGGLGGYM LGSAMSRPIIHFGSDYEDRY YRENMHRYPNQVYYRPMDEY SNQNNFVHDCVNITIKQHTV TTTTKGENFTETDVKMMERV VEQMCITQYKRESQAYYQRG SS

Data sets:
Data typeCount
13C chemical shifts461
15N chemical shifts148
1H chemical shifts1281

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human prion protein with E219K protective polymorphism1

Entities:

Entity 1, Human prion protein with E219K protective polymorphism 142 residues - 16169.169 Da.

1   GLYGLNGLYGLYGLYTHRHISSERGLNTRP
2   ASNLYSPROSERLYSPROLYSTHRASNMET
3   LYSHISMETALAGLYALAALAALAALAGLY
4   ALAVALVALGLYGLYLEUGLYGLYTYRMET
5   LEUGLYSERALAMETSERARGPROILEILE
6   HISPHEGLYSERASPTYRGLUASPARGTYR
7   TYRARGGLUASNMETHISARGTYRPROASN
8   GLNVALTYRTYRARGPROMETASPGLUTYR
9   SERASNGLNASNASNPHEVALHISASPCYS
10   VALASNILETHRILELYSGLNHISTHRVAL
11   THRTHRTHRTHRLYSGLYGLUASNPHETHR
12   GLUTHRASPVALLYSMETMETGLUARGVAL
13   VALGLUGLNMETCYSILETHRGLNTYRLYS
14   ARGGLUSERGLNALATYRTYRGLNARGGLY
15   SERSER

Related Database Links:

BMRB 15676 16743 16757 17714 17756 17757 18426 18550 19268 4379 4402 4434 4620 4641 4736
PDB 1E1G 1E1J 1E1P 1E1S 1E1U 1E1W 1FKC 1FO7 1HJM 1HJN 1I4M 1QLX 1QLZ 1QM0 1QM1 1QM2 1QM3 2K1D 2KUN 2LEJ 2LFT 2LSB 2LV1 2M8T 2W9E 3HAF 3HAK 3HEQ 3HER 3HES 3HJ5 3HJX 4DGI 4KML 4N9O
DBJ BAA00011 BAF62360 BAG32276 BAG32277 BAG32278
EMBL CAA58442 CAG46836 CAG46869
GB AAA19664 AAA60182 AAA68632 AAA68633 AAB59442
REF NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592
SP P04156 P40252 P61766 P61767 P61768
AlphaFold P04156 P40252 P61766 P61767 P61768

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