BMRB Entry 17775

Title:
Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer
Deposition date:
2011-07-10
Original release date:
2011-08-16
Authors:
Coles, Murray; Ferris, Hedda; Hulko, Michael; Martin, Joerg; Lupas, Andrei
Citation:

Citation: Ferris, Hedda; Dunin-Horkawicz, Stanislaw; Hornig, Nora; Hulko, Michael; Martin, Jorg; Schultz, Joachim; Zeth, Kornelius; Lupas, Andrei; Coles, Murray. "Mechanism of regulation of receptor histidine kinases."  Structure 20, 56-66 (2012).
PubMed: 22244755

Assembly members:

Assembly members:
HAMP-DHp, polymer, 112 residues, 12738.506 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30b

Data sets:
Data typeCount
13C chemical shifts456
15N chemical shifts112
1H chemical shifts758

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HAMP-DHp, chain 11
2HAMP-DHp, chain 21

Entities:

Entity 1, HAMP-DHp, chain 1 112 residues - 12738.506 Da.

The start methionine represents a cloning artifact

1   METSERTHRILETHRARGPROILEILEGLU
2   LEUSERASNTHRALAASPLYSILEALAGLU
3   GLYASNLEUGLUALAGLUVALPROHISGLN
4   ASNARGALAASPGLUILEGLYILELEUALA
5   LYSSERILEGLUARGLEUARGARGSERLEU
6   LYSGLNLEUALAASPASPARGTHRLEULEU
7   METALAGLYVALSERHISASPLEUARGTHR
8   PROLEUTHRARGILEARGLEUALATHRGLU
9   METMETSERGLUGLNASPGLYTYRLEUALA
10   GLUSERILEASNLYSASPILEGLUGLUCYS
11   ASNALAILEILEGLUGLNPHEILEASPTYR
12   LEUARG

Related Database Links:

UNP P0AEJ4 O28769
BMRB 17776
PDB 2LFR 2LFS 3ZCC 3ZRV 3ZRW 3ZRX 4CTI
GB ESL47213
AlphaFold O28769 Q2M769

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks