BMRB Entry 17765

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PDB ID: 2lfn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17765
MolProbity Validation Chart

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Title:
Identification of the key regions that drive functional amyloid formation by the fungal hydrophobin EAS
Deposition date:
2011-07-06
Original release date:
2012-01-24
Authors:
Macindoe, Ingrid; Kwan, Ann; Morris, Vanessa; Mackay, Joel; Sunde, Margie
Citation:

Citation: Macindoe, Ingrid; Kwan, Ann; Ren, Qin; Morris, Vanessa; Yang, Wenrong; Mackay, Joel; Sunde, Margaret. "Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS."  Proc. Natl. Acad. Sci. U.S.A. 109, E804-E811 (2012).
PubMed: 22308366

Assembly members:

Assembly members:
entity, polymer, 68 residues, 6728.595 Da.

Natural source:

Natural source:   Common Name: Neurospora crassa   Taxonomy ID: 5141   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Neurospora crassa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHUE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SATTIGPNTCSIDDYKPYCC QSMSGSASLGCVVGVIGSQC GASVKCCKDDVTNTGNSGLI INAANCVA

Data sets:
Data typeCount
1H chemical shifts420

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1EAS1

Entities:

Entity 1, EAS 68 residues - 6728.595 Da.

Residue 1 represents a cloning artifact.

1   SERALATHRTHRILEGLYPROASNTHRCYS
2   SERILEASPASPTYRLYSPROTYRCYSCYS
3   GLNSERMETSERGLYSERALASERLEUGLY
4   CYSVALVALGLYVALILEGLYSERGLNCYS
5   GLYALASERVALLYSCYSCYSLYSASPASP
6   VALTHRASNTHRGLYASNSERGLYLEUILE
7   ILEASNALAALAASNCYSVALALA

Related Database Links:

BMRB 15863
PDB 2K6A 2LFN