BMRB Entry 17711
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PDB ID: 2leh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17711
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Sarachan, Kathryn; Valentine, Kathleen; Gupta, Kushol; Moorman, Veronica; Gledhill Jr, John; Bernens, Matthew; Tommos, Cecilia; Wand, A. Joshua; Van Duyne, Gregory. "Solution structure of the core SMN-Gemin2 complex." Biochem J. 445, 361-370 (2012).
PubMed: 22607171
Assembly members:
gemin2, polymer, 189 residues, 21609.596 Da.
smn, polymer, 26 residues, 2863.100 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKS987
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 914 |
| 15N chemical shifts | 206 |
| 1H chemical shifts | 1425 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gemin2 | 1 |
| 2 | smn | 2 |
Entities:
Entity 1, gemin2 189 residues - 21609.596 Da.
The C-terminal residues Leu-Glu-Tyr are a non-native cloning artifact.
| 1 | GLY | TYR | SER | PRO | THR | LEU | GLN | TRP | GLN | GLN | ||||
| 2 | GLN | GLN | VAL | ALA | GLN | PHE | SER | THR | VAL | ARG | ||||
| 3 | GLN | ASN | VAL | ASN | LYS | HIS | ARG | SER | HIS | TRP | ||||
| 4 | LYS | SER | GLN | GLN | LEU | ASP | SER | ASN | VAL | THR | ||||
| 5 | MET | PRO | LYS | SER | GLU | ASP | GLU | GLU | GLY | TRP | ||||
| 6 | LYS | LYS | PHE | CYS | LEU | GLY | GLU | LYS | LEU | CYS | ||||
| 7 | ALA | ASP | GLY | ALA | VAL | GLY | PRO | ALA | THR | ASN | ||||
| 8 | GLU | SER | PRO | GLY | ILE | ASP | TYR | VAL | GLN | ILE | ||||
| 9 | GLY | PHE | PRO | PRO | LEU | LEU | SER | ILE | VAL | SER | ||||
| 10 | ARG | MET | ASN | GLN | ALA | THR | VAL | THR | SER | VAL | ||||
| 11 | LEU | GLU | TYR | LEU | SER | ASN | TRP | PHE | GLY | GLU | ||||
| 12 | ARG | ASP | PHE | THR | PRO | GLU | LEU | GLY | ARG | TRP | ||||
| 13 | LEU | TYR | ALA | LEU | LEU | ALA | CYS | LEU | GLU | LYS | ||||
| 14 | PRO | LEU | LEU | PRO | GLU | ALA | HIS | SER | LEU | ILE | ||||
| 15 | ARG | GLN | LEU | ALA | ARG | ARG | CYS | SER | GLU | VAL | ||||
| 16 | ARG | LEU | LEU | VAL | ASP | SER | LYS | ASP | ASP | GLU | ||||
| 17 | ARG | VAL | PRO | ALA | LEU | ASN | LEU | LEU | ILE | CYS | ||||
| 18 | LEU | VAL | SER | ARG | TYR | PHE | ASP | GLN | ARG | ASP | ||||
| 19 | LEU | ALA | ASP | GLU | PRO | SER | LEU | GLU | TYR |
Entity 2, smn 26 residues - 2863.100 Da.
| 1 | GLY | GLN | SER | ASP | ASP | SER | ASP | ILE | TRP | ASP | ||||
| 2 | ASP | THR | ALA | LEU | ILE | LYS | ALA | TYR | ASP | LYS | ||||
| 3 | ALA | VAL | ALA | SER | PHE | LYS |
Related Database Links:
| PDB | 2LEH 3S6N |
| DBJ | BAB03509 BAG36665 BAD51989 BAE02007 BAE26061 BAE39854 BAF82358 |
| EMBL | CAC16117 CAA73356 CAB41938 CAH89989 CDQ72355 |
| GB | AAB82297 AAI04969 AIC50129 EAW65825 EAW65829 AAA64505 AAA66242 AAB58318 AAB96377 AAC01747 |
| REF | NP_001009183 NP_003607 XP_001092707 XP_001145975 XP_002807275 NP_000335 NP_001003226 NP_001009328 NP_001124207 NP_001124942 |
| SP | O14893 O02771 O35876 P97801 Q16637 Q4R4F8 |
| PRF | 2208336A |
| AlphaFold | O14893 O02771 O35876 P97801 Q16637 Q4R4F8 |
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