BMRB Entry 17686

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PDB ID: 2ldy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17686
MolProbity Validation Chart

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Title:
Solution structure of the RMM-CTD domains of human LINE-1 ORF1p
Deposition date:
2011-06-03
Original release date:
2012-02-22
Authors:
Coles, Murray; Truffault, Vincent
Citation:

Citation: Khazina, Elena; Truffault, Vincent; Buttner, Regina; Schmidt, Steffen; Coles, Murray; Weichenrieder, Oliver. "Trimeric structure and flexibility of the L1ORF1 protein in human L1 retrotransposition."  Nat. Struct. Mol. Biol. 18, 1006-1014 (2011).
PubMed: 21822284

Assembly members:

Assembly members:
L1ORF1p, polymer, 182 residues, 20688.154 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
L1ORF1p: MGNLRLIGVPESDVENGTKL ENTLQDIIQENFPNLARQAN VQIQEIQRTPQRYSSRRATP RHIIVRFTKVEMKEKMLRAA REKGRVTLKGKPIRLTVDLS AETLQARREWGPIFNILKEK NFQPRISYPAKLSFISEGEI KYFIDKQMLRDFVTTRPALK ELLKEALNMERNNRYQHHHH HH

Data sets:
Data typeCount
13C chemical shifts697
15N chemical shifts153
1H chemical shifts1236

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1L1ORF1p1

Entities:

Entity 1, L1ORF1p 182 residues - 20688.154 Da.

Mutations R255M and P156G were for cloning purposes. Residues 331-336 are a hexahistidine purification tag

1   METGLYASNLEUARGLEUILEGLYVALPRO
2   GLUSERASPVALGLUASNGLYTHRLYSLEU
3   GLUASNTHRLEUGLNASPILEILEGLNGLU
4   ASNPHEPROASNLEUALAARGGLNALAASN
5   VALGLNILEGLNGLUILEGLNARGTHRPRO
6   GLNARGTYRSERSERARGARGALATHRPRO
7   ARGHISILEILEVALARGPHETHRLYSVAL
8   GLUMETLYSGLULYSMETLEUARGALAALA
9   ARGGLULYSGLYARGVALTHRLEULYSGLY
10   LYSPROILEARGLEUTHRVALASPLEUSER
11   ALAGLUTHRLEUGLNALAARGARGGLUTRP
12   GLYPROILEPHEASNILELEULYSGLULYS
13   ASNPHEGLNPROARGILESERTYRPROALA
14   LYSLEUSERPHEILESERGLUGLYGLUILE
15   LYSTYRPHEILEASPLYSGLNMETLEUARG
16   ASPPHEVALTHRTHRARGPROALALEULYS
17   GLULEULEULYSGLUALALEUASNMETGLU
18   ARGASNASNARGTYRGLNHISHISHISHIS
19   HISHIS

Related Database Links:

PDB 2LDY 2W7A 2YKO 2YKP 2YKQ
EMBL CJZ86355
GB AAB59367 AAC51262 AAC51278 AAD39214 AAL50636
PRF 1901303A
SP Q9UN81
AlphaFold Q9UN81

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks