BMRB Entry 17685

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PDB ID: 2lwx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17685
MolProbity Validation Chart

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Title:
Solution structure of the C-terminal Pdr1 activating domain of Zuo1
Deposition date:
2011-06-02
Original release date:
2012-10-01
Authors:
Volkman, B.; Waltner, J.; Peterson, F.
Citation:

Citation: Ducett, Jeanette; Peterson, Francis; Hoover, Lindsey; Prunuske, Amy; Volkman, Brian; Craig, Elizabeth. "Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription."  J. Mol. Biol. 425, 19-31 (2013).
PubMed: 23036859

Assembly members:

Assembly members:
Zuo1, polymer, 108 residues, 11836.323 Da.

Natural source:

Natural source:   Common Name: yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Zuo1: MRGSHHHHHHHHGSENLYFQ GSKAAKKKNKRAIRNSAKEA DYFGDADKATTIDEQVGLIV DSLNDEELVSTADKIKANAA GAKEVLKESAKTIVDSGKLP SSLLSYFV

Data sets:
Data typeCount
13C chemical shifts373
15N chemical shifts93
1H chemical shifts616

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Zuo11

Entities:

Entity 1, Zuo1 108 residues - 11836.323 Da.

The N-terminal residues 'MRGSHHHHHHHHGSENLYFQGS' are a cloning artifact.

1   METARGGLYSERHISHISHISHISHISHIS
2   HISHISGLYSERGLUASNLEUTYRPHEGLN
3   GLYSERLYSALAALALYSLYSLYSASNLYS
4   ARGALAILEARGASNSERALALYSGLUALA
5   ASPTYRPHEGLYASPALAASPLYSALATHR
6   THRILEASPGLUGLNVALGLYLEUILEVAL
7   ASPSERLEUASNASPGLUGLULEUVALSER
8   THRALAASPLYSILELYSALAASNALAALA
9   GLYALALYSGLUVALLEULYSGLUSERALA
10   LYSTHRILEVALASPSERGLYLYSLEUPRO
11   SERSERLEULEUSERTYRPHEVAL

Related Database Links:

PDB 2LWX
DBJ GAA23651
EMBL CAY80044
GB AHY79619 AJP39050 AJR76370 AJR76866 AJR77364

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