BMRB Entry 17685

Title:
Solution structure of the C-terminal Pdr1 activating domain of Zuo1
Deposition date:
2011-06-02
Original release date:
2012-10-01
Authors:
Volkman, B.; Waltner, J.; Peterson, F.
Citation:

Citation: Ducett, Jeanette; Peterson, Francis; Hoover, Lindsey; Prunuske, Amy; Volkman, Brian; Craig, Elizabeth. "Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription."  J. Mol. Biol. 425, 19-31 (2013).
PubMed: 23036859

Assembly members:

Assembly members:
Zuo1, polymer, 108 residues, 11836.323 Da.

Natural source:

Natural source:   Common Name: yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Data sets:
Data typeCount
13C chemical shifts373
15N chemical shifts93
1H chemical shifts616

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Zuo11

Entities:

Entity 1, Zuo1 108 residues - 11836.323 Da.

The N-terminal residues 'MRGSHHHHHHHHGSENLYFQGS' are a cloning artifact.

1   METARGGLYSERHISHISHISHISHISHIS
2   HISHISGLYSERGLUASNLEUTYRPHEGLN
3   GLYSERLYSALAALALYSLYSLYSASNLYS
4   ARGALAILEARGASNSERALALYSGLUALA
5   ASPTYRPHEGLYASPALAASPLYSALATHR
6   THRILEASPGLUGLNVALGLYLEUILEVAL
7   ASPSERLEUASNASPGLUGLULEUVALSER
8   THRALAASPLYSILELYSALAASNALAALA
9   GLYALALYSGLUVALLEULYSGLUSERALA
10   LYSTHRILEVALASPSERGLYLYSLEUPRO
11   SERSERLEULEUSERTYRPHEVAL

Samples:

sample: Zuo1, [U-100% 13C; U-100% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESY (AROMATIC)sampleisotropicsample_conditions_1
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_15N-separated_NOESYsampleisotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker Avance II 600 MHz

Related Database Links:

PDB
DBJ GAA23651
EMBL CAY80044
GB AHY79619 AJP39050 AJR76370 AJR76866 AJR77364

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks