BMRB Entry 17653

Title:
solution structure of the mSin3A PAH3-SAP30 SID complex
Deposition date:
2011-05-16
Original release date:
2011-10-26
Authors:
Xie, Tao; He, Yuan; Korkeamaki, Hanna; Zhang, Yongbo; Imhoff, Rebecca; Lohi, Olli; Radhakrishnan, Ishwar
Citation:

Citation: Xie, Tao; He, Yuan; Korkeamaki, Hanna; Zhang, Yongbo; Imhoff, Rebecca; Lohi, Olli; Radhakrishnan, Ishwar. "Structure of the 30-kDa Sin3-associated protein (SAP30) in complex with the mammalian Sin3A corepressor and its role in nucleic acid binding."  J. Biol. Chem. 286, 27814-27824 (2011).
PubMed: 21676866

Assembly members:

Assembly members:
SAP30, polymer, 94 residues, 10546.846 Da.
entity_2, polymer, 75 residues, 8674.021 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCSG23

Data sets:
Data typeCount
13C chemical shifts699
15N chemical shifts174
1H chemical shifts1086

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SAP301
2PAH32

Entities:

Entity 1, SAP30 94 residues - 10546.846 Da.

Residues 1-3 are from non-native tag. This is the SID domain of SAP30 protein.

1   SERASNALAGLYSERASPASPASPGLYGLY
2   ASPSERPROVALGLNASPILEASPTHRPRO
3   GLUVALASPLEUTYRGLNLEUGLNVALASN
4   THRLEUARGARGTYRLYSARGHISPHELYS
5   LEUPROTHRARGPROGLYLEUASNLYSALA
6   GLNLEUVALGLUILEVALGLYCYSHISPHE
7   LYSSERILEPROVALASNGLULYSASPTHR
8   LEUTHRCYSPHEILETYRSERVALARGASN
9   ASPLYSASNLYSSERASPLEULYSALAASP
10   SERGLYVALHIS

Entity 2, PAH3 75 residues - 8674.021 Da.

Residues 1-3 are from non-native tag. This is the PAH3 domain of mSIN3A.

1   SERASNALASERLYSHISGLYVALGLYTHR
2   GLUSERLEUPHEPHEASPLYSVALARGLYS
3   ALALEUARGSERALAGLUALATYRGLUASN
4   PHELEUARGCYSLEUVALILEPHEASNGLN
5   GLUVALILESERARGALAGLULEUVALGLN
6   LEUVALSERPROPHELEUGLYLYSPHEPRO
7   GLULEUPHEASNTRPPHELYSASNPHELEU
8   GLYTYRLYSGLUSER

Related Database Links:

PDB 2LD7
DBJ BAB27273 BAC40543 BAD90217
GB AAC26007 AAI32082 AAI32088 EDL28616 EDL28617 AAA69772 AAA69773 AAA89119 AAB01610 AAH52716
REF NP_068560 XP_005607821 XP_006253148 XP_008512731 XP_008770398 NP_001102231 NP_001103820 NP_001103821 NP_035508 XP_001491398
SP O88574 Q60520
TPG DAA17551
AlphaFold O88574 Q60520

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks