BMRB Entry 17643
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PDB ID: 2mwg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17643
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Jurk, Marcel; Dorn, Matthias; Schmieder, Peter. "Blue flickers of hope: secondary structure, dynamics, and putative dimerization interface of the blue-light receptor YtvA from Bacillus subtilis." Biochemistry 50, 8163-8171 (2011).
PubMed: 21851109
Assembly members:
YtvA, polymer, 261 residues, 29500 Da.
FMN, non-polymer, 456.344 Da.
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30EK/LIC
Entity Sequences (FASTA):
YtvA: GASFQSFGIPGQLEVIKKAL
DHVRVGVVITDPALEDNPIV
YVNQGFVQMTGYETEEILGK
NCRFLQGKHTDPAEVDNIRT
ALQNKEPVTVQIQNYKKDGT
MFWNELNIDPMEIEDKTYFV
GIQNDITKQKEYEKLLEDSL
TEITALSTPIVPIRNGISAL
PLVGNLTEERFNSIVCTLTN
ILSTSKDDYLIIDLSGLAQV
NEQTADQIFKLSHLLKLTGT
ELIITGIKPELAMKMNKLDA
NFSSLKTYSNVKDAVKVLPI
M
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 782 |
| 15N chemical shifts | 242 |
| 1H chemical shifts | 347 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Monomer 1 | 1 |
| 2 | Monomer 2 | 1 |
| 3 | Cofactor 1 | 2 |
| 4 | Cofactor 2 | 2 |
Entities:
Entity 1, Monomer 1 261 residues - 29500 Da.
Gly1 is a remainder of the proteolytic cleavage site
| 1 | GLY | ALA | SER | PHE | GLN | SER | PHE | GLY | ILE | PRO | ||||
| 2 | GLY | GLN | LEU | GLU | VAL | ILE | LYS | LYS | ALA | LEU | ||||
| 3 | ASP | HIS | VAL | ARG | VAL | GLY | VAL | VAL | ILE | THR | ||||
| 4 | ASP | PRO | ALA | LEU | GLU | ASP | ASN | PRO | ILE | VAL | ||||
| 5 | TYR | VAL | ASN | GLN | GLY | PHE | VAL | GLN | MET | THR | ||||
| 6 | GLY | TYR | GLU | THR | GLU | GLU | ILE | LEU | GLY | LYS | ||||
| 7 | ASN | CYS | ARG | PHE | LEU | GLN | GLY | LYS | HIS | THR | ||||
| 8 | ASP | PRO | ALA | GLU | VAL | ASP | ASN | ILE | ARG | THR | ||||
| 9 | ALA | LEU | GLN | ASN | LYS | GLU | PRO | VAL | THR | VAL | ||||
| 10 | GLN | ILE | GLN | ASN | TYR | LYS | LYS | ASP | GLY | THR | ||||
| 11 | MET | PHE | TRP | ASN | GLU | LEU | ASN | ILE | ASP | PRO | ||||
| 12 | MET | GLU | ILE | GLU | ASP | LYS | THR | TYR | PHE | VAL | ||||
| 13 | GLY | ILE | GLN | ASN | ASP | ILE | THR | LYS | GLN | LYS | ||||
| 14 | GLU | TYR | GLU | LYS | LEU | LEU | GLU | ASP | SER | LEU | ||||
| 15 | THR | GLU | ILE | THR | ALA | LEU | SER | THR | PRO | ILE | ||||
| 16 | VAL | PRO | ILE | ARG | ASN | GLY | ILE | SER | ALA | LEU | ||||
| 17 | PRO | LEU | VAL | GLY | ASN | LEU | THR | GLU | GLU | ARG | ||||
| 18 | PHE | ASN | SER | ILE | VAL | CYS | THR | LEU | THR | ASN | ||||
| 19 | ILE | LEU | SER | THR | SER | LYS | ASP | ASP | TYR | LEU | ||||
| 20 | ILE | ILE | ASP | LEU | SER | GLY | LEU | ALA | GLN | VAL | ||||
| 21 | ASN | GLU | GLN | THR | ALA | ASP | GLN | ILE | PHE | LYS | ||||
| 22 | LEU | SER | HIS | LEU | LEU | LYS | LEU | THR | GLY | THR | ||||
| 23 | GLU | LEU | ILE | ILE | THR | GLY | ILE | LYS | PRO | GLU | ||||
| 24 | LEU | ALA | MET | LYS | MET | ASN | LYS | LEU | ASP | ALA | ||||
| 25 | ASN | PHE | SER | SER | LEU | LYS | THR | TYR | SER | ASN | ||||
| 26 | VAL | LYS | ASP | ALA | VAL | LYS | VAL | LEU | PRO | ILE | ||||
| 27 | MET |
Entity 2, Cofactor 1 - C17 H21 N4 O9 P - 456.344 Da.
| 1 | FMN |
Related Database Links:
| DBJ | BAI86545 BAM54284 BAM59113 GAK82103 |
| EMBL | CAB15012 CCU59545 CEI58264 CEJ78686 CJR42913 |
| GB | AAC00382 ABN71355 ACR16779 ACR43777 ADJ00051 |
| REF | NP_390912 WP_003229169 WP_004399022 WP_014477717 WP_014480616 |
| SP | O34627 |
| AlphaFold | O34627 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks