BMRB Entry 17623

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PDB ID: 2yh1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17623
MolProbity Validation Chart

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Title:
MODEL OF HUMAN U2AF65 TANDEM RRM1 AND RRM2 DOMAINS WITH EIGHT-SITE URIDINE BINDING
Deposition date:
2011-05-04
Original release date:
2011-08-03
Authors:
Mackereth, C.; Madl, T.; Simon, B.; Zanier, K.; Gasch, A.; Sattler, Michael
Citation:

Citation: Mackereth, C.; Madl, T.; Bonnal, S.; Simon, B.; Zanier, K.; Gasch, A.; Rybin, V.; Valcarcel, J.; Sattler, Michael. "Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF"  Nature ., .-..

Assembly members:

Assembly members:
HUMAN_U2AF65, polymer, 198 residues, 21491.534 Da.
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3', polymer, 9 residues, 2774.53744 Da.

Natural source:

Natural source:   Common Name: HUMAN   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: unknown

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HUMAN_U2AF65: GAMARRLYVGNIPFGITEEA MMDFFNAQMRLGGLTQAPGN PVLAVQINQDKNFAFLEFRS VDETTQAMAFDGIIFQGQSL KIRRPHDYQPLPGMSENPSV YVPGVVSTVVPDSAHKLFIG GLPNYLNDDQVKELLTSFGP LKAFNLVKDSATGLSKGYAF CEYVDINVTDQAIAGLNGMQ LGDKKLLVQRASVGAKNA
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3': UUUUUUUUU

Data sets:
Data typeCount
13C chemical shifts375
15N chemical shifts184
1H chemical shifts914

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HUMAN U2AF651
25'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3'2

Entities:

Entity 1, HUMAN U2AF65 198 residues - 21491.534 Da.

1   GLYALAMETALAARGARGLEUTYRVALGLY
2   ASNILEPROPHEGLYILETHRGLUGLUALA
3   METMETASPPHEPHEASNALAGLNMETARG
4   LEUGLYGLYLEUTHRGLNALAPROGLYASN
5   PROVALLEUALAVALGLNILEASNGLNASP
6   LYSASNPHEALAPHELEUGLUPHEARGSER
7   VALASPGLUTHRTHRGLNALAMETALAPHE
8   ASPGLYILEILEPHEGLNGLYGLNSERLEU
9   LYSILEARGARGPROHISASPTYRGLNPRO
10   LEUPROGLYMETSERGLUASNPROSERVAL
11   TYRVALPROGLYVALVALSERTHRVALVAL
12   PROASPSERALAHISLYSLEUPHEILEGLY
13   GLYLEUPROASNTYRLEUASNASPASPGLN
14   VALLYSGLULEULEUTHRSERPHEGLYPRO
15   LEULYSALAPHEASNLEUVALLYSASPSER
16   ALATHRGLYLEUSERLYSGLYTYRALAPHE
17   CYSGLUTYRVALASPILEASNVALTHRASP
18   GLNALAILEALAGLYLEUASNGLYMETGLN
19   LEUGLYASPLYSLYSLEULEUVALGLNARG
20   ALASERVALGLYALALYSASNALA

Entity 2, 5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3' 9 residues - 2774.53744 Da.

1   UUUUUUUUU

Related Database Links:

UNP U2AF2_HUMAN
BMRB 17622
PDB 2YH0 2YH1
DBJ BAC37309 BAG70075 BAG70201 BAI45669
EMBL CAA45409 CAA45874 CAA45875 CAF97922
GB AAH07487 AAH08740 AAH30574 AAH43071 AAH44032
PRF 1805352A
REF NP_001001217 NP_001012496 NP_001025127 NP_001068804 NP_001080595
SP P26368 P26369
TPG DAA19368
AlphaFold Q96HC5 P26368 P26369

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