BMRB Entry 17606
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17606
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gong, Weibin; Zhou, Tao; Mo, Jinjin; Perrett, Sarah; Wang, Jinfeng; Feng, Yingang. "Structural Insight into Recognition of Methylated Histone Tails by Retinoblastoma-binding Protein 1." J. Biol. Chem. 287, 8531-8540 (2012).
PubMed: 22247551
Assembly members:
entity, polymer, 76 residues, 9082.347 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30a
Entity Sequences (FASTA):
entity: EDMEPCLTGTKVKVKYGRGK
TQKIYEASIKSTEIDDGEVL
YLVHYYGWNVRYDEWVKADR
IIWPLDKGLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 327 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 476 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RBBP1 | 1 |
Entities:
Entity 1, RBBP1 76 residues - 9082.347 Da.
| 1 | GLU | ASP | MET | GLU | PRO | CYS | LEU | THR | GLY | THR | ||||
| 2 | LYS | VAL | LYS | VAL | LYS | TYR | GLY | ARG | GLY | LYS | ||||
| 3 | THR | GLN | LYS | ILE | TYR | GLU | ALA | SER | ILE | LYS | ||||
| 4 | SER | THR | GLU | ILE | ASP | ASP | GLY | GLU | VAL | LEU | ||||
| 5 | TYR | LEU | VAL | HIS | TYR | TYR | GLY | TRP | ASN | VAL | ||||
| 6 | ARG | TYR | ASP | GLU | TRP | VAL | LYS | ALA | ASP | ARG | ||||
| 7 | ILE | ILE | TRP | PRO | LEU | ASP | LYS | GLY | LEU | GLU | ||||
| 8 | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: RBBP1, [U-95% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 5 mM; H2O 90%; D2O 10%
sample_2: entity, [U-95% 13C; U-95% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.4 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| GB | AAB25833 AAB25834 AAB25835 AAB28543 AAI72363 |
| REF | NP_001101499 NP_001231108 NP_001243510 NP_002883 NP_075376 |
| SP | P29374 |
| TPG | DAA25277 |
| AlphaFold | P29374 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks