BMRB Entry 17603

Name: Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant
Published: 2011-06-01

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PDB ID: 2lc9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17603
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant

Deposition date:

2011-04-26

Original release date:

2011-06-01

Authors:

Bouvignies, Guillaume; Vallurupalli, Pramodh; Hansen, D. Flemming; Correia, Bruno; Lange, Oliver; Bah, Alaji; Vernon, Robert; Dahlquist, Frederick; Baker, David; Kay, Lewis

Citation:

Citation: Bouvignies, Guillaume; Vallurupalli, Pramodh; Hansen, D. Flemming; Correia, Bruno; Lange, Oliver; Bah, Alaji; Vernon, Robert; Dahlquist, Frederick; Baker, David; Kay, Lewis. "Solution structure of a minor and transiently formed state of a T4 lysozyme mutant." Nature 477, 111-114 (2011).
PubMed: 21857680

Assembly members:

Assembly members:
T4_L99A/G113A/R119P, polymer, 164 residues, 18540.383 Da.

Natural source:

Natural source: Common Name: T4 Phage Taxonomy ID: not available Superkingdom: Viruses Kingdom: not available Genus/species: T4 Phage

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-29B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
T4_L99A/G113A/R119P: MNIFEMLRIDEGLRLKIYKD TEGYYTIGIGHLLTKSPSLN AAKSELDKAIGRNTNGVITK DEAEKLFNQDVDAAVRGILR NAKLKPVYDSLDAVRRAAAI NMVFQMGETGVAAFTNSLPM LQQKRWDEAAVNLAKSRWYN QTPNRAKRVITTFRTGTWDA YKNL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	474
15N chemical shifts	159
1H chemical shifts	327

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T4_L99A/G113A/R119P	1

Entities:

Entity 1, T4_L99A/G113A/R119P 164 residues - 18540.383 Da.

Mutant of T4 Phage Lysozyme

1

MET

ASN

ILE

PHE

GLU

MET

LEU

ARG

ILE

ASP

2

GLU

GLY

LEU

ARG

LEU

LYS

ILE

TYR

LYS

ASP

3

THR

GLU

GLY

TYR

THR

ILE

GLY

ILE

GLY

4

HIS

LEU

THR

LYS

SER

PRO

SER

LEU

ASN

5

ALA

LYS

SER

GLU

LEU

ASP

LYS

ALA

ILE

6

GLY

ARG

ASN

THR

ASN

GLY

VAL

ILE

THR

LYS

7

ASP

GLU

ALA

GLU

LYS

LEU

PHE

ASN

GLN

ASP

8

VAL

ASP

ALA

VAL

ARG

GLY

ILE

LEU

ARG

9

ASN

ALA

LYS

LEU

LYS

PRO

VAL

TYR

ASP

SER

10

LEU

ASP

ALA

VAL

ARG

ALA

ILE

11

ASN

MET

VAL

PHE

GLN

MET

GLY

GLU

THR

GLY

12

VAL

ALA

PHE

THR

ASN

SER

LEU

PRO

MET

13

LEU

GLN

LYS

ARG

TRP

ASP

GLU

ALA

14

VAL

ASN

LEU

ALA

LYS

SER

ARG

TRP

TYR

ASN

15

GLN

THR

PRO

ASN

ARG

ALA

LYS

ARG

VAL

ILE

16

THR

PHE

ARG

THR

GLY

THR

TRP

ASP

ALA

17

TYR

LYS

ASN

LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
JMOD C'Cg	sample_1	isotropic	sample_conditions_2
JMDO NCg	sample_1	isotropic	sample_conditions_2

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CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 17603

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: