BMRB Entry 17586

Name: Rv0020c_FHA Structure
Published: 2011-10-26

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PDB ID: 2lc1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17586
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Rv0020c_FHA Structure

Deposition date:

2011-04-11

Original release date:

2011-10-26

Authors:

Barthe, Philippe; Cohen-Gonsaud, Martin; Roumestand, Christian

Citation:

Citation: Roumestand, Christian; Leiba, Jade; Galophe, Nathalie; Margeat, Emmanuel; Padilla, Andre; Bessin, Yannick; Barthe, Philippe; Molle, Virginie; Cohen-Gonsaud, Martin. "Structural Insight into the Mycobacterium tuberculosis Rv0020c Protein and Its Interaction with the PknB Kinase." Structure 19, 1525-1534 (2011).
PubMed: 22000520

Assembly members:

Assembly members:
Rv0020c_FHA, polymer, 100 residues, 10888.060 Da.

Natural source:

Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b-tev

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rv0020c_FHA: GHGSAGTSVTLQLDDGSGRT YQLREGSNIIGRGQDAQFRL PDTGVSRRHLEIRWDGQVAL LADLNSTNGTTVNNAPVQEW QLADGDVIRLGHSEIIVRMH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	287
15N chemical shifts	114
1H chemical shifts	673

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rv0020c_FHA	1

Entities:

Entity 1, Rv0020c_FHA 100 residues - 10888.060 Da.

1	GLY	HIS	GLY	SER	ALA	GLY	THR	SER	VAL	THR
2	LEU	GLN	LEU	ASP	ASP	GLY	SER	GLY	ARG	THR
3	TYR	GLN	LEU	ARG	GLU	GLY	SER	ASN	ILE	ILE
4	GLY	ARG	GLY	GLN	ASP	ALA	GLN	PHE	ARG	LEU
5	PRO	ASP	THR	GLY	VAL	SER	ARG	ARG	HIS	LEU
6	GLU	ILE	ARG	TRP	ASP	GLY	GLN	VAL	ALA	LEU
7	LEU	ALA	ASP	LEU	ASN	SER	THR	ASN	GLY	THR
8	THR	VAL	ASN	ASN	ALA	PRO	VAL	GLN	GLU	TRP
9	GLN	LEU	ALA	ASP	GLY	ASP	VAL	ILE	ARG	LEU
10	GLY	HIS	SER	GLU	ILE	ILE	VAL	ARG	MET	HIS

Samples:

15N: Rv0020c_FHA, [U-15N], 0.5 ± 0.01 mM

13C-15N: Rv0020c_FHA, [U-13C; U-15N], 0.5 ± 0.01 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	15N	isotropic	sample_conditions_1
3D 1H-15N TOCSY	15N	isotropic	sample_conditions_1
3D HNCA	13C-15N	isotropic	sample_conditions_1
3D CBCA(CO)NH	13C-15N	isotropic	sample_conditions_1
3D HNCACB	13C-15N	isotropic	sample_conditions_1
3D HNCO	13C-15N	isotropic	sample_conditions_1
3D HCACO	13C-15N	isotropic	sample_conditions_1

Software:

GIFA v4.44, Delsuc - processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TALOS+ v1.2009.0721.18, Cornilescu, Delaglio and Bax - angle prediction

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 500 MHz

PDB	2LC1 3OUN 3PO8 3POA
DBJ	BAH24322 BAL63843 BAN29123 BAQ03850
EMBL	CAL70034 CCC25094 CCC42360 CCC62613 CCE35563
GB	AAK44245 AAS02340 ABK67403 ABQ71740 ABR04363
REF	NP_214534 NP_853690 WP_003400376 WP_003872107 WP_003876793
SP	P71590
AlphaFold	P71590

1	GLY	HIS	GLY	SER	ALA	GLY	THR	SER	VAL	THR
2	LEU	GLN	LEU	ASP	ASP	GLY	SER	GLY	ARG	THR
3	TYR	GLN	LEU	ARG	GLU	GLY	SER	ASN	ILE	ILE
4	GLY	ARG	GLY	GLN	ASP	ALA	GLN	PHE	ARG	LEU
5	PRO	ASP	THR	GLY	VAL	SER	ARG	ARG	HIS	LEU
6	GLU	ILE	ARG	TRP	ASP	GLY	GLN	VAL	ALA	LEU
7	LEU	ALA	ASP	LEU	ASN	SER	THR	ASN	GLY	THR
8	THR	VAL	ASN	ASN	ALA	PRO	VAL	GLN	GLU	TRP
9	GLN	LEU	ALA	ASP	GLY	ASP	VAL	ILE	ARG	LEU
10	GLY	HIS	SER	GLU	ILE	ILE	VAL	ARG	MET	HIS

1	GLY	HIS	GLY	SER	ALA	GLY	THR	SER	VAL	THR
2	LEU	GLN	LEU	ASP	ASP	GLY	SER	GLY	ARG	THR
3	TYR	GLN	LEU	ARG	GLU	GLY	SER	ASN	ILE	ILE
4	GLY	ARG	GLY	GLN	ASP	ALA	GLN	PHE	ARG	LEU
5	PRO	ASP	THR	GLY	VAL	SER	ARG	ARG	HIS	LEU
6	GLU	ILE	ARG	TRP	ASP	GLY	GLN	VAL	ALA	LEU
7	LEU	ALA	ASP	LEU	ASN	SER	THR	ASN	GLY	THR
8	THR	VAL	ASN	ASN	ALA	PRO	VAL	GLN	GLU	TRP
9	GLN	LEU	ALA	ASP	GLY	ASP	VAL	ILE	ARG	LEU
10	GLY	HIS	SER	GLU	ILE	ILE	VAL	ARG	MET	HIS