BMRB Entry 17575

Title:
solution structure of the C domain of Rv0899 from mycobacterium tuberculosis
Deposition date:
2011-04-06
Original release date:
2012-01-04
Authors:
Yao, Yong; Marassi, Francesca
Citation:

Citation: Yao, Yong; Barghava, Neha; Kim, Johnny; Niederweis, Michael; Marassi, Francesca. "Molecular Structure and Peptidoglycan Recognition of Mycobacterium tuberculosis ArfA (Rv0899)."  J. Mol. Biol. 416, 208-220 (2012).
PubMed: 22206986

Assembly members:

Assembly members:
entity, polymer, 137 residues, 14052.831 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21b

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts129
1H chemical shifts795

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C domain of Rv08991

Entities:

Entity 1, C domain of Rv0899 137 residues - 14052.831 Da.

1   GLYGLNALAPROPROGLYPROPROALASER
2   GLYPROCYSALAASPLEUGLNSERALAILE
3   ASNALAVALTHRGLYGLYPROILEALAPHE
4   GLYASNASPGLYALASERLEUILEPROALA
5   ALATYRGLUILELEUASNARGVALALAASP
6   LYSLEULYSALACYSPROASPALAARGVAL
7   THRILEASNGLYTYRTHRASPASNTHRGLY
8   SERGLUGLYILEASNILEPROLEUSERALA
9   GLNARGALALYSILEVALALAASPTYRLEU
10   VALALAARGGLYVALALAGLYASPHISILE
11   ALATHRVALGLYLEUGLYSERVALASNPRO
12   ILEALASERASNALATHRPROGLUGLYARG
13   ALALYSASNARGARGVALGLUILEVALVAL
14   ASNHISHISHISHISHISHIS

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 0.5 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16237 17863
PDB
DBJ BAH25212 BAL64801 BAQ04819 GAA44658
EMBL CAL70937 CCC25980 CCC43237 CCC63509 CCE36433
GB AAK45169 ABI54278 ABQ72638 ABR05261 ACT26182
REF NP_215414 NP_854580 WP_003404684 WP_003915129 WP_014000491
SP A1KH31 P65594 P9WIU4 P9WIU5
AlphaFold P65594 A1KH31 P9WIU4 P9WIU5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks