BMRB Entry 17536

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PDB ID: 2las
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17536
MolProbity Validation Chart

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Title:
Molecular Determinants of Paralogue-Specific SUMO-SIM Recognition
Deposition date:
2011-03-20
Original release date:
2011-12-14
Authors:
Namanja, Andrew; Li, Yi-Jia; Su, Yang; Wong, Steven; Lu, Jingjun; Colson, Loren; Wu, Chenggang; Li, Shawn; Chen, Yuan
Citation:

Citation: Namanja, Andrew; Li, Yi-Jia; Su, Yang; Wong, Steven; Lu, Jingjun; Colson, Loren; Wu, Chenggang; Li, Shawn; Chen, Yuan. "Insights into High Affinity Small Ubiquitin-like Modifier (SUMO) Recognition by SUMO-interacting Motifs (SIMs) Revealed by a Combination of NMR and Peptide Array Analysis."  J. Biol. Chem. 287, 3231-3240 (2012).
PubMed: 22147707

Assembly members:

Assembly members:
SUMO1, polymer, 97 residues, 9099.442 Da.
M-IR2_peptide, polymer, 13 residues, 1616.870 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SUMO1: MSDQEAKPSTEDLGDKKEGE YIKLKVIGQDSSEIHFKVKM TTHLKKLKESYCQRQGVPMN SLRFLFEGQRIADNHTPKEL GMEEEDVIEVYQEQTGG
M-IR2_peptide: DNEIEVIIVWEKK

Data sets:
Data typeCount
13C chemical shifts225
15N chemical shifts92
1H chemical shifts198

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1SUMO11
2M-IR22

Entities:

Entity 1, SUMO1 97 residues - 9099.442 Da.

1   METSERASPGLNGLUALALYSPROSERTHR
2   GLUASPLEUGLYASPLYSLYSGLUGLYGLU
3   TYRILELYSLEULYSVALILEGLYGLNASP
4   SERSERGLUILEHISPHELYSVALLYSMET
5   THRTHRHISLEULYSLYSLEULYSGLUSER
6   TYRCYSGLNARGGLNGLYVALPROMETASN
7   SERLEUARGPHELEUPHEGLUGLYGLNARG
8   ILEALAASPASNHISTHRPROLYSGLULEU
9   GLYMETGLUGLUGLUASPVALILEGLUVAL
10   TYRGLNGLUGLNTHRGLYGLY

Entity 2, M-IR2 13 residues - 1616.870 Da.

1   ASPASNGLUILEGLUVALILEILEVALTRP
2   GLULYSLYS

Related Database Links:

BMRB 16611 25299
PDB 1A5R 1TGZ 1WYW 1Y8R 1Z5S 2ASQ 2BF8 2G4D 2IO2 2IY0 2IY1 2KQS 2LAS 2MW5 2PE6 2UYZ 2VRR 3KYC 3KYD 3RZW 3UIP 4WJN 4WJO 4WJP 4WJQ
DBJ BAB22172 BAB27379 BAB93477 BAC40739 BAE35024
EMBL CAA67898 CAG31129 CAG46944 CAG46953 CAH92616
GB AAB39999 AAB40388 AAB40390 AAC39959 AAC50733
REF NP_001005781 NP_001005782 NP_001009672 NP_001030535 NP_001106146
SP A7WLH8 P63165 P63166 Q2EF74 Q5E9D1
TPG DAA32560
AlphaFold A7WLH8 P63165 P63166 Q2EF74 Q5E9D1 Q59FH7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks