BMRB Entry 17503

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17503

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Title:
Staphylococcus aureus FusB
Deposition date:
2011-03-03
Original release date:
2012-02-17
Authors:
Cox, Georgina; O'Neill, Alex; Thompson, Gary; Kalverda, Arnout; Homans, Steve; Tomlinson, Jennifer; Edwards, Thomas; Jenkins, Huw
Citation:

Citation: Cox, Georgina; Thompson, Gary; Jenkins, Huw; Peske, Frank; Savelsbergh, Andreas; Rodnina, Marina; Wintermeyer, Wolfgang; Homans, Steve; Edwards, Thomas; O'Neill, Alexander. "Ribosome clearance by FusB-type proteins mediates resistance to the antibiotic fusidic acid"  Proc. Natl. Acad. Sci. U. S. A. 109, 2102-2107 (2102).
PubMed: 22308410

Assembly members:

Assembly members:
fusb, polymer, 233 residues, 27325.3922 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a-fusB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fusb: MGSSHHHHHHSSGLVPNGSH MKTMIYPHQYNYIRSVILRL KNVYKTVNDKETVKVIQSET YNDINEIFGHIDDDIEESLK VLMNIRLSNKEIEAILNKFL EYVVPFELPSPQKLQKVFKK VKKIKIPQFEEYDLKVSSFV GWNELASNRKYIIYYDEKKQ LKGLYGEISNQVVKGFCTIC NKESNVSLFMKKSKTNSDGQ YVKKGDYICRDSIHCNKQLT DINQFYNFIDKLD

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts169
1H chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1fusb1

Entities:

Entity 1, fusb 233 residues - 27325.3922 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROASNGLYSERHIS
3   METLYSTHRMETILETYRPROHISGLNTYR
4   ASNTYRILEARGSERVALILELEUARGLEU
5   LYSASNVALTYRLYSTHRVALASNASPLYS
6   GLUTHRVALLYSVALILEGLNSERGLUTHR
7   TYRASNASPILEASNGLUILEPHEGLYHIS
8   ILEASPASPASPILEGLUGLUSERLEULYS
9   VALLEUMETASNILEARGLEUSERASNLYS
10   GLUILEGLUALAILELEUASNLYSPHELEU
11   GLUTYRVALVALPROPHEGLULEUPROSER
12   PROGLNLYSLEUGLNLYSVALPHELYSLYS
13   VALLYSLYSILELYSILEPROGLNPHEGLU
14   GLUTYRASPLEULYSVALSERSERPHEVAL
15   GLYTRPASNGLULEUALASERASNARGLYS
16   TYRILEILETYRTYRASPGLULYSLYSGLN
17   LEULYSGLYLEUTYRGLYGLUILESERASN
18   GLNVALVALLYSGLYPHECYSTHRILECYS
19   ASNLYSGLUSERASNVALSERLEUPHEMET
20   LYSLYSSERLYSTHRASNSERASPGLYGLN
21   TYRVALLYSLYSGLYASPTYRILECYSARG
22   ASPSERILEHISCYSASNLYSGLNLEUTHR
23   ASPILEASNGLNPHETYRASNPHEILEASP
24   LYSLEUASP

Samples:

FusB_1: fusb, [[U-95% 13C; U-95% 15N; U-95% 2H]], 0.3 mM; H2O 90%; D2O 10%; Tris-HCl 20 mM; NaCl 300 mM; DTT 1 mM

15N-val-FusB: fusb, [U-15N]-Val, 0.78 mM; H2O 90%; D2O 10%; Tris-HCl 20 mM; NaCl 300 mM; DTT 1 mM

15N-ile-FusB: fusb, [U-15N]-Ile, 0.72 mM; H2O 90%; D2O 10%; Tris-HCl 20 mM; NaCl 300 mM; DTT 1 mM

15N-tyr-FusB: fusb, [U-15N]-Tyr, 0.68 mM; H2O 90%; D2O 10%; Tris-HCl 20 mM; NaCl 300 mM; DTT 1 mM

15N-leu-FusB: fusb, [U-15N]-Leu, 0.8 mM; H2O 90%; D2O 10%; Tris-HCl 20 mM; NaCl 300 mM; DTT 1 mM

FusB_buffer: ionic strength: 0.300 M; pH: 8.000; pressure: 1.000 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAFusB_1isotropicFusB_buffer
3D HN(CO)CAFusB_1isotropicFusB_buffer
hncaco (H[N[ca[CO]]])FusB_1isotropicFusB_buffer
3D HNCOFusB_1isotropicFusB_buffer
3D HNCACBFusB_1isotropicFusB_buffer
hncocacb (H[N[co[{CA|ca[C]}]]])FusB_1isotropicFusB_buffer
2D 1H-15N HSQC/HMQCFusB_1isotropicFusB_buffer
2D 1H-15N HSQC/HMQC15N-val-FusBisotropicFusB_buffer
2D 1H-15N HSQC/HMQC15N-ile-FusBisotropicFusB_buffer
2D 1H-15N HSQC/HMQC15N-tyr-FusBisotropicFusB_buffer
2D 1H-15N HSQC/HMQC15N-leu-FusBisotropicFusB_buffer

Software:

ANALYSIS v1.0, CCPN - assignment

ANALYSIS v2.1, CCPN - assignment

NMRPipe v3.0, Frank Delaglio, Stephan Grzesiek, Guang Zhu, Geerten W. Vuister, John Pfeifer, and Ad Bax - processing spectra

NMR spectrometers:

  • Varian UnityInova 749 MHz
  • Varian UnityInova 600 MHz
  • Varian UnityInova 500 MHz

Related Database Links:

PDB
DBJ BAO09119 BAO09144
EMBL CAJ43426 CAL23838 CQA10371 CQD31768 CRI24900
GB AAL12234 AAN07146 ABB82610 AEU08355 AEU08384
REF NP_932197 WP_000855537 WP_032491632 YP_009078429 YP_009080413

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks