BMRB Entry 17494

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PDB ID: 2l9z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17494
MolProbity Validation Chart

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Title:
Zinc knuckle in PRDM4
Deposition date:
2011-02-26
Original release date:
2011-06-01
Authors:
Briknarova, Klara; Atwater, Daniel; Glicken, Jessica; Maynard, Stacy; Ness, Tara
Citation:

Citation: Briknarova, Klara; Atwater, Daniel; Glicken, Jessica; Maynard, Stacy; Ness, Tara. "The PR/SET domain in PRDM4 is preceded by a zinc knuckle."  Proteins 79, 2341-2345 (2011).
PubMed: 21604305

Assembly members:

Assembly members:
PRDM4(366-402), polymer, 39 residues, 4316.912 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PRDM4(366-402): GSKENMATLFTIWCTLCDRA YPSDCPEHGPVTFVPDTPI

Data sets:
Data typeCount
15N chemical shifts36
1H chemical shifts258

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PRDM4(366-402)1
2ZINC ION2

Entities:

Entity 1, PRDM4(366-402) 39 residues - 4316.912 Da.

G364 and S365 are cloning artefacts

1   GLYSERLYSGLUASNMETALATHRLEUPHE
2   THRILETRPCYSTHRLEUCYSASPARGALA
3   TYRPROSERASPCYSPROGLUHISGLYPRO
4   VALTHRPHEVALPROASPTHRPROILE

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2L9Z
DBJ BAG10863 BAG51692
EMBL CAB61401 CAH92945
GB AAD55249 AAH35581 ABM82163 ABM85348 AIC50813
REF NP_001126735 NP_001244489 NP_036538 XP_001162965 XP_002807184
SP Q5R5M1 Q9UKN5
AlphaFold Q5R5M1 Q9UKN5

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