BMRB Entry 17412
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PDB ID: 2l8j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17412
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Rozenknop, Alexis; Rogov, Vladimir; Rogova, Natalia Yu; Lohr, Frank; Guntert, Peter; Dikic, Ivan; Dotsch, Volker. "Characterization of the Interaction of GABARAPL-1 with the LIR Motif of NBR1." J. Mol. Biol. 410, 477-487 (2011).
PubMed: 21620860
Assembly members:
GABARAPL-1, polymer, 119 residues, 14268.327 Da.
NBR1-LIR, polymer, 18 residues, 1840.027 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T1
Entity Sequences (FASTA):
GABARAPL-1: GSPEFKFQYKEDHPFEYRKK
EGEKIRKKYPDRVPVIVEKA
PKARVPDLDKRKYLVPSDLT
VGQFYFLIRKRIHLRPEDAL
FFFVNNTIPPTSATMGQLYE
DNHEEDYFLYVAYSDESVY
NBR1-LIR: GAMGSASSEDYIIILPES
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 506 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 881 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GABARAPL-1 | 1 |
| 2 | NBR1-LIR | 2 |
Entities:
Entity 1, GABARAPL-1 119 residues - 14268.327 Da.
Residues 1-5 represent a non-native cloning artefact
| 1 | GLY | SER | PRO | GLU | PHE | LYS | PHE | GLN | TYR | LYS | ||||
| 2 | GLU | ASP | HIS | PRO | PHE | GLU | TYR | ARG | LYS | LYS | ||||
| 3 | GLU | GLY | GLU | LYS | ILE | ARG | LYS | LYS | TYR | PRO | ||||
| 4 | ASP | ARG | VAL | PRO | VAL | ILE | VAL | GLU | LYS | ALA | ||||
| 5 | PRO | LYS | ALA | ARG | VAL | PRO | ASP | LEU | ASP | LYS | ||||
| 6 | ARG | LYS | TYR | LEU | VAL | PRO | SER | ASP | LEU | THR | ||||
| 7 | VAL | GLY | GLN | PHE | TYR | PHE | LEU | ILE | ARG | LYS | ||||
| 8 | ARG | ILE | HIS | LEU | ARG | PRO | GLU | ASP | ALA | LEU | ||||
| 9 | PHE | PHE | PHE | VAL | ASN | ASN | THR | ILE | PRO | PRO | ||||
| 10 | THR | SER | ALA | THR | MET | GLY | GLN | LEU | TYR | GLU | ||||
| 11 | ASP | ASN | HIS | GLU | GLU | ASP | TYR | PHE | LEU | TYR | ||||
| 12 | VAL | ALA | TYR | SER | ASP | GLU | SER | VAL | TYR |
Entity 2, NBR1-LIR 18 residues - 1840.027 Da.
Residues 1-4 and 18 represent a non-native cloning artefact
| 1 | GLY | ALA | MET | GLY | SER | ALA | SER | SER | GLU | ASP | ||||
| 2 | TYR | ILE | ILE | ILE | LEU | PRO | GLU | SER |
Related Database Links:
| PDB | 2L8J 2R2Q |
| DBJ | BAA95100 BAB27950 BAB29146 BAB29690 BAB31345 |
| EMBL | CAB66611 CAG38511 CAH89636 CAL38063 CAL38067 |
| GB | AAH04602 AAH09309 AAH24706 AAH28315 AAH72921 |
| REF | NP_001025652 NP_001028788 NP_001037759 NP_001085553 NP_001088067 |
| SP | P60518 Q0VGK0 Q5BIZ2 Q5RF21 Q6GQ27 |
| TPG | DAA29267 |
| AlphaFold | P60518 Q0VGK0 Q5BIZ2 Q5RF21 Q6GQ27 |
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CSV: Backbone
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