BMRB Entry 17390

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PDB ID: 2l82
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17390
MolProbity Validation Chart

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Title:
Solution NMR Structure of de novo designed protein, P-loop NTPase fold, Northeast Structural Genomics Consortium Target OR32
Deposition date:
2010-12-31
Original release date:
2011-01-24
Authors:
Liu, Gaohua; Koga, Nobuyasu; Koga, Rie; Xiao, Rong; Hamilton, Keith; Janjua, Haleema; Tong, Saichu; Acton, Thomas; Everett, John; Baker, David; Montelione, Gaetano
Citation:

Citation: Koga, N.; Koga, R.; Liu, G.; Castellanos, J.; Montelione, G.; Baker, D.. "Role of backbone strain in de novo design of complex alpha/beta protein structures"  Nat. Commun. 12, 3921-3921 (2021).
PubMed: 34168113

Assembly members:

Assembly members:
OR32, polymer, 162 residues, 19701.238 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OR32: MSQIFVVFSSDPEILKEIVR EIKRQGVRVVLLYSDQDEKR RRERLEEFEKQGVDVRTVED KEDFRENIREIWERYPQLDV VVIVTTDDKEWIKDFIEEAK ERGVEVFVVYNNKDDDRRKE AQQEFRSDGVDVRTVSDKEE LIEQVRRFVRKVGSLEHHHH HH

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts173
1H chemical shifts1181

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1OR321

Entities:

Entity 1, OR32 162 residues - 19701.238 Da.

1   METSERGLNILEPHEVALVALPHESERSER
2   ASPPROGLUILELEULYSGLUILEVALARG
3   GLUILELYSARGGLNGLYVALARGVALVAL
4   LEULEUTYRSERASPGLNASPGLULYSARG
5   ARGARGGLUARGLEUGLUGLUPHEGLULYS
6   GLNGLYVALASPVALARGTHRVALGLUASP
7   LYSGLUASPPHEARGGLUASNILEARGGLU
8   ILETRPGLUARGTYRPROGLNLEUASPVAL
9   VALVALILEVALTHRTHRASPASPLYSGLU
10   TRPILELYSASPPHEILEGLUGLUALALYS
11   GLUARGGLYVALGLUVALPHEVALVALTYR
12   ASNASNLYSASPASPASPARGARGLYSGLU
13   ALAGLNGLNGLUPHEARGSERASPGLYVAL
14   ASPVALARGTHRVALSERASPLYSGLUGLU
15   LEUILEGLUGLNVALARGARGPHEVALARG
16   LYSVALGLYSERLEUGLUHISHISHISHIS
17   HISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks