BMRB Entry 17377

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17377
MolProbity Validation Chart

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Title:
Solution structure of the MFS-bound Sans CEN2 peptide
Deposition date:
2010-12-22
Original release date:
2011-02-14
Authors:
Pan, Lifeng; Wu, Lin; Wei, Zhiyi; Zhang, Mingjie
Citation:

Citation: Wu, Lin; Pan, Lifeng; Wei, Zhiyi; Zhang, Mingjie. "Structure of MyTH4-FERM Domains in Myosin VIIa Tail Bound to Cargo."  Science 331, 757-760 (2011).
PubMed: 21311020

Assembly members:

Assembly members:
entity, polymer, 11 residues, 1315.396 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: EELPWDELDLG

Data sets:
Data typeCount
1H chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sans CEN21

Entities:

Entity 1, Sans CEN2 11 residues - 1315.396 Da.

1   GLUGLULEUPROTRPASPGLULEUASPLEU
2   GLY

Samples:

sample_1: sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

PIPP, Garrett - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz