BMRB Entry 17376

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17376

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Title:
Backbone Assignment of Human Proliferating Cell Nuclear Antigen Complexed with a 12 Amino Acids Peptide from the C-terminal Region of p21 (WAF1/CIP1)
Deposition date:
2010-12-22
Original release date:
2012-11-26
Authors:
Lopez-Alonso, Jorge; Sanchez, Ricardo; Blanco, Francisco; Campos-Olivas, Ramon
Citation:

Citation: De Biasio, Alfredo; Campos-Olivas, Ramon; Sanchez, Ricardo; Lopez-Alonso, Jorge; Pantoja-Uceda, David; Merino, Nekane; Villate, Maider; Martin-Garcia, Jose; Castillo, Francisco; Luque, Irene; Blanco, Francisco. "Proliferating Cell Nuclear Antigen (PCNA) Interactions in Solution Studied by NMR"  PLoS One 7, e48390-e48390 (2012).
PubMed: 23139781

Assembly members:

Assembly members:
hPCNA, polymer, 264 residues, Formula weight is not available
p2112, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Rosetta BL21 (DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hPCNA: GPHMFEARLVQGSILKKVLE ALKDLINEACWDISSSGVNL QSMDSSHVSLVQLTLRSEGF DTYRCDRNLAMGVNLTSMSK ILKCAGNEDIITLRAEDNAD TLALVFEAPNQEKVSDYEMK LMDLDVEQLGIPEQEYSCVV KMPSGEFARICRDLSHIGDA VVISCAKDGVKFSASGELGN GNIKLSQTSNVDKEEEAVTI EMNEPVQLTFALRYLNFFTK ATPLSSTVTLSMSADVPLVV EYKIADMGHLKYYLAPKIED EEGS
p2112: KRRQTSMTDFYH

Data sets:
Data typeCount
13C chemical shifts676
15N chemical shifts258
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hPCNA monomer 11
2hPCNA monomer 21
3hPCNA monomer 31
4P2112 molecule 12
5P2112 molecule 22
6P2112 molecule 32

Entities:

Entity 1, hPCNA monomer 1 264 residues - Formula weight is not available

Residues -3 to -1 are a non-native tag. The native sequence starts at residue number 4: MF...

1   GLYPROHISMETPHEGLUALAARGLEUVAL
2   GLNGLYSERILELEULYSLYSVALLEUGLU
3   ALALEULYSASPLEUILEASNGLUALACYS
4   TRPASPILESERSERSERGLYVALASNLEU
5   GLNSERMETASPSERSERHISVALSERLEU
6   VALGLNLEUTHRLEUARGSERGLUGLYPHE
7   ASPTHRTYRARGCYSASPARGASNLEUALA
8   METGLYVALASNLEUTHRSERMETSERLYS
9   ILELEULYSCYSALAGLYASNGLUASPILE
10   ILETHRLEUARGALAGLUASPASNALAASP
11   THRLEUALALEUVALPHEGLUALAPROASN
12   GLNGLULYSVALSERASPTYRGLUMETLYS
13   LEUMETASPLEUASPVALGLUGLNLEUGLY
14   ILEPROGLUGLNGLUTYRSERCYSVALVAL
15   LYSMETPROSERGLYGLUPHEALAARGILE
16   CYSARGASPLEUSERHISILEGLYASPALA
17   VALVALILESERCYSALALYSASPGLYVAL
18   LYSPHESERALASERGLYGLULEUGLYASN
19   GLYASNILELYSLEUSERGLNTHRSERASN
20   VALASPLYSGLUGLUGLUALAVALTHRILE
21   GLUMETASNGLUPROVALGLNLEUTHRPHE
22   ALALEUARGTYRLEUASNPHEPHETHRLYS
23   ALATHRPROLEUSERSERTHRVALTHRLEU
24   SERMETSERALAASPVALPROLEUVALVAL
25   GLUTYRLYSILEALAASPMETGLYHISLEU
26   LYSTYRTYRLEUALAPROLYSILEGLUASP
27   GLUGLUGLYSER

Entity 2, P2112 molecule 1 12 residues - Formula weight is not available

1   LYSARGARGGLNTHRSERMETTHRASPPHE
2   TYRHIS

Related Database Links:

BMRB 15501 17375
PDB 1AXC 1U76 1U7B 1UL1 1VYJ 1VYM 1W60 2ZVK 2ZVL 2ZVM 3P87 3TBL 3VKX 3WGW 4D2G 4RJF
DBJ BAB28355 BAB28557 BAC40240 BAE30230 BAE30376
EMBL CAA40938 CAA68261 CAG38740 CAG46598
GB AAA35736 AAA60040 AAG10077 AAG37435 AAH00491
REF NP_001029666 NP_001233697 NP_001253019 NP_001274649 NP_001278854
SP P04961 P12004 P17918 P57761 P61258
TPG DAA23435
AlphaFold P04961 P12004 P17918 P57761 P61258

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks