BMRB Entry 17374

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17374
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignments for ADF/Cofilin from Trypanosoma brucei
Deposition date:
2010-12-22
Original release date:
2011-01-06
Authors:
Dai, Kun; Yuan, Guangfa; Liao, Shanhui; Zhang, Jiahai; Tu, Xiaoming
Citation:

Citation: Dai, Kun; Yuan, Guangfa; Liao, Shanhui; Zhang, Jiahai; Tu, Xiaoming. "(1)H, (13)C and (15)N resonance assignments for a putative ADF/Cofilin from Trypanosoma brucei."  Biomol. NMR Assignments 5, 249-251 (2011).
PubMed: 21523437

Assembly members:

Assembly members:
ADF_Cofilin, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ADF_Cofilin: MGHHHHHHMAMSGVSVADEC VTALNDLRHKKSRYVIMHIV DQKSIAVKTIGERGANFDQF IEAIDKNVPCYAAFDFEYTT NDGPRDKLILISWNPDSGAP RTKMLYSSSRDALVPLTQGF QGIQANDASGLDFEEISRKV KSNR

Data sets:
Data typeCount
13C chemical shifts527
15N chemical shifts130
1H chemical shifts812

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ADF/Cofilin from Trypanosoma brucei1

Entities:

Entity 1, ADF/Cofilin from Trypanosoma brucei 144 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISMETALA
2   METSERGLYVALSERVALALAASPGLUCYS
3   VALTHRALALEUASNASPLEUARGHISLYS
4   LYSSERARGTYRVALILEMETHISILEVAL
5   ASPGLNLYSSERILEALAVALLYSTHRILE
6   GLYGLUARGGLYALAASNPHEASPGLNPHE
7   ILEGLUALAILEASPLYSASNVALPROCYS
8   TYRALAALAPHEASPPHEGLUTYRTHRTHR
9   ASNASPGLYPROARGASPLYSLEUILELEU
10   ILESERTRPASNPROASPSERGLYALAPRO
11   ARGTHRLYSMETLEUTYRSERSERSERARG
12   ASPALALEUVALPROLEUTHRGLNGLYPHE
13   GLNGLYILEGLNALAASNASPALASERGLY
14   LEUASPPHEGLUGLUILESERARGLYSVAL
15   LYSSERASNARG

Samples:

sample_1: ADF/Cofilin, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 100 mM; sodium phosphate 20 mM; EDTA 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
EMBL CBH10242
GB AAX81027 AAZ10549
REF XP_011772532 XP_844108

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks