BMRB Entry 17344

Click here to enlarge.

PDB ID: 2l75
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17344
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of CHD4-PHD2 in complex with H3K9me3
Deposition date:
2010-12-02
Original release date:
2011-02-01
Authors:
Mansfield, Robyn; Kwan, Ann; Mackay, Joel
Citation:

Citation: Mansfield, Robyn; Musselman, Catherine; Kwan, Ann; Oliver, Samuel; Garske, Adam; Davrazou, Foteini; Denu, John; Kutateladze, Tatiana; Mackay, Joel. "Plant Homeodomain (PHD) Fingers of CHD4 Are Histone H3-binding Modules with Preference for Unmodified H3K4 and Methylated H3K9."  J. Biol. Chem. 286, 11779-11791 (2011).
PubMed: 21278251

Assembly members:

Assembly members:
CHD4-PHD2, polymer, 61 residues, Formula weight is not available
H3K9me3, polymer, 14 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2TE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CHD4-PHD2: GPLGSDHHMEFCRVCKDGGE LLCCDTCPSSYHIHCLNPPL PEIPNGEWLCPRCTCPALKG K
H3K9me3: ARTKQTARXSTGGY

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts61
1H chemical shifts484

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CHD4-PHD21
2H3K9me32
3ZINC ION_13
4ZINC ION_23

Entities:

Entity 1, CHD4-PHD2 61 residues - Formula weight is not available

Non-native N-terminal GPLGS due to cleavage of expression tag.

1   GLYPROLEUGLYSERASPHISHISMETGLU
2   PHECYSARGVALCYSLYSASPGLYGLYGLU
3   LEULEUCYSCYSASPTHRCYSPROSERSER
4   TYRHISILEHISCYSLEUASNPROPROLEU
5   PROGLUILEPROASNGLYGLUTRPLEUCYS
6   PROARGCYSTHRCYSPROALALEULYSGLY
7   LYS

Entity 2, H3K9me3 14 residues - Formula weight is not available

C-terminal Tyr is not part of native H3 sequence, but added for concentration determination by A280.

1   ALAARGTHRLYSGLNTHRALAARGM3LSER
2   THRGLYGLYTYR

Entity 3, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 1MM2 2L75
DBJ BAE32198
REF XP_012406166 XP_013226542
SWS P68431

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks