BMRB Entry 17323

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PDB ID: 2l6u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17323
MolProbity Validation Chart

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Title:
Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A
Deposition date:
2010-11-24
Original release date:
2010-12-22
Authors:
Eletsky, Alexander; Ryuechan, William; Sukumaran, Dinesh; Shastry, Ritu; Ciccosanti, Colleen; Janjua, Haleema; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Eletsky, Alexander; Ruyechan, William; Xiao, Rong; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25."  J. Struct. Funct. Genomics 12, 159-166 (2011).
PubMed: 21785987

Assembly members:

Assembly members:
HR6188A, polymer, 163 residues, 18637.756 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 14-15C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR6188A: MGHHHHHHSHGQQSVSNKLL AWSGVLEWQEKPKPASVDAN TKLTRSLPCQVYVNHGENLK TEQWPQKLIMQLIPQQLLTT LGPLFRNSRMVQFHFTNKDL ESLKGLYRIMGNGFAGCVHF PHTAPCEVRVLMLLYSSKKK IFMGLIPYDQSGFVNGIRQV ITN

Data sets:
Data typeCount
13C chemical shifts721
15N chemical shifts172
1H chemical shifts1160

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1HR6188A1

Entities:

Entity 1, HR6188A 163 residues - 18637.756 Da.

residues 381-390 represent a non-native affinity tag

1   METGLYHISHISHISHISHISHISSERHIS
2   GLYGLNGLNSERVALSERASNLYSLEULEU
3   ALATRPSERGLYVALLEUGLUTRPGLNGLU
4   LYSPROLYSPROALASERVALASPALAASN
5   THRLYSLEUTHRARGSERLEUPROCYSGLN
6   VALTYRVALASNHISGLYGLUASNLEULYS
7   THRGLUGLNTRPPROGLNLYSLEUILEMET
8   GLNLEUILEPROGLNGLNLEULEUTHRTHR
9   LEUGLYPROLEUPHEARGASNSERARGMET
10   VALGLNPHEHISPHETHRASNLYSASPLEU
11   GLUSERLEULYSGLYLEUTYRARGILEMET
12   GLYASNGLYPHEALAGLYCYSVALHISPHE
13   PROHISTHRALAPROCYSGLUVALARGVAL
14   LEUMETLEULEUTYRSERSERLYSLYSLYS
15   ILEPHEMETGLYLEUILEPROTYRASPGLN
16   SERGLYPHEVALASNGLYILEARGGLNVAL
17   ILETHRASN

Related Database Links:

BMRB 17139
PDB 2KY6 2L23 2L6U 2XNF
DBJ BAE29135 BAF82149 BAG51583 BAG61008
EMBL CAB66680 CAE84581
GB AAG15589 AAH21333 AAH24312 AAH31138 AAH65297
REF NP_001075914 NP_001163897 NP_083641 NP_112235 XP_001115473
SP A2VE44 Q71SY5 Q8VCB2
TPG DAA19611
AlphaFold A2VE44 Q71SY5 Q8VCB2

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks