BMRB Entry 17278
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17278
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Shukla, Vaibhav Kumar; Pathak, Prem Prakash; Yadav, Rahul; Srivastava, Shubhra; Jain, Anupam; Pulavarti, S.V.S.R. Krishna; Arora, Ashish. "NMR assignment of actin depolymerizing and dynamics regulatory protein from Toxoplasma gondii" Biomol. NMR Assignments ., .-..
Assembly members:
TgADF, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: Toxoplasma gondii Taxonomy ID: 5811 Superkingdom: Eukaryota Kingdom: not available Genus/species: Toxoplasma gondii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b+
Entity Sequences (FASTA):
TgADF: MASGMGVDENCVARFNELKI
RKTVKWIVFKIENTKIVVEK
DGKGNADEFRGALPANDCRF
GVYDCGNKIQFVLWCPDNAP
VKPRMTYASSKDALLKKLDG
ATAVALEAHEMGDLAPLA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 478 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 721 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Monomer | 1 |
Entities:
Entity 1, Monomer 118 residues - Formula weight is not available
| 1 | MET | ALA | SER | GLY | MET | GLY | VAL | ASP | GLU | ASN | ||||
| 2 | CYS | VAL | ALA | ARG | PHE | ASN | GLU | LEU | LYS | ILE | ||||
| 3 | ARG | LYS | THR | VAL | LYS | TRP | ILE | VAL | PHE | LYS | ||||
| 4 | ILE | GLU | ASN | THR | LYS | ILE | VAL | VAL | GLU | LYS | ||||
| 5 | ASP | GLY | LYS | GLY | ASN | ALA | ASP | GLU | PHE | ARG | ||||
| 6 | GLY | ALA | LEU | PRO | ALA | ASN | ASP | CYS | ARG | PHE | ||||
| 7 | GLY | VAL | TYR | ASP | CYS | GLY | ASN | LYS | ILE | GLN | ||||
| 8 | PHE | VAL | LEU | TRP | CYS | PRO | ASP | ASN | ALA | PRO | ||||
| 9 | VAL | LYS | PRO | ARG | MET | THR | TYR | ALA | SER | SER | ||||
| 10 | LYS | ASP | ALA | LEU | LEU | LYS | LYS | LEU | ASP | GLY | ||||
| 11 | ALA | THR | ALA | VAL | ALA | LEU | GLU | ALA | HIS | GLU | ||||
| 12 | MET | GLY | ASP | LEU | ALA | PRO | LEU | ALA |
Samples:
sample_1: potassium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; DTT 1 mM; AEBSF protease inhibitor 1 mM; H2O 95%; D2O 5%; TgADF, [U-100% 13C; U-100% 15N], 0.8 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| HBCB(CGCD)HD | sample_1 | isotropic | sample_conditions_1 |
| HBCB(CGCDCE)HE | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA, Keller and Wuthrich - chemical shift assignment
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| GB | AAC47717 EPR57074 EPT31140 ESS28466 KFG28323 |
| REF | XP_002371565 |
| TPE | CEL72722 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks