BMRB Entry 17257

Name: NMR structure of the Xanthomonas VirB7
Published: 2011-05-19

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PDB ID: 2l4w
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17257
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the Xanthomonas VirB7

Deposition date:

2010-10-15

Original release date:

2011-05-19

Authors:

Souza, Diorge; Farah, Chuck; Salinas, Roberto

Citation:

Citation: Souza, Diorge; Andrade, Maxuel; Alvarez-Martinez, Cristina; Arantes, Guilherme; Farah, Chuck; Salinas, Roberto. "A Component of the Xanthomonadaceae Type IV Secretion System Combines a VirB7 Motif with a N0 Domain Found in Outer Membrane Transport Proteins." PLoS Pathog. 7, .-. (2011).
PubMed: 21589901

Assembly members:

Assembly members:
VirB7-Xac2622, polymer, 120 residues, 12871.521 Da.

Natural source:

Natural source: Common Name: Xanthomonas axonopodis pv. citri Taxonomy ID: 92829 Superkingdom: bacteria Kingdom: not available Genus/species: Xanthomonas axonopodis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VirB7-Xac2622: GSHMTKPAPDFGGRWKHVNH FDEAPTEIPLYTSYTYQATP MDGTLKTMLERWAADSNMQL SYNLPSDYTLIGPVSAISTT SVQQAATELSAVYAAQGVSV SVSANKLLVQPVPVSSGAKL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	518
15N chemical shifts	122
1H chemical shifts	804

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VirB7-Xac2622 monomer	1

Entities:

Entity 1, VirB7-Xac2622 monomer 120 residues - 12871.521 Da.

The first four residues (20-23) are derived from the non-native affinity tag. Residues 24-139 correspond to the predicted soluble region of VirB7-Xac2622. The native protein would be anchored via a lipidated cys residue at position 22. The native residues 1-23 are not present in this construct.

1	GLY	SER	HIS	MET	THR	LYS	PRO	ALA	PRO	ASP
2	PHE	GLY	GLY	ARG	TRP	LYS	HIS	VAL	ASN	HIS
3	PHE	ASP	GLU	ALA	PRO	THR	GLU	ILE	PRO	LEU
4	TYR	THR	SER	TYR	THR	TYR	GLN	ALA	THR	PRO
5	MET	ASP	GLY	THR	LEU	LYS	THR	MET	LEU	GLU
6	ARG	TRP	ALA	ALA	ASP	SER	ASN	MET	GLN	LEU
7	SER	TYR	ASN	LEU	PRO	SER	ASP	TYR	THR	LEU
8	ILE	GLY	PRO	VAL	SER	ALA	ILE	SER	THR	THR
9	SER	VAL	GLN	GLN	ALA	ALA	THR	GLU	LEU	SER
10	ALA	VAL	TYR	ALA	ALA	GLN	GLY	VAL	SER	VAL
11	SER	VAL	SER	ALA	ASN	LYS	LEU	LEU	VAL	GLN
12	PRO	VAL	PRO	VAL	SER	SER	GLY	ALA	LYS	LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC J-modulated	sample_1	anisotropic	sample_conditions_2

PDB	2L4W 3OV5
EMBL	CAJ24488 CCF70408 CCG39329 CDF62143 CDN21695
GB	AAM37471 AGH78108 AGI08427 AJD69220 AJY82742
REF	WP_005914237 WP_033836637 WP_039430902 WP_042598437

BMRB Entry 17257

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	HIS	MET	THR	LYS	PRO	ALA	PRO	ASP
2	PHE	GLY	GLY	ARG	TRP	LYS	HIS	VAL	ASN	HIS
3	PHE	ASP	GLU	ALA	PRO	THR	GLU	ILE	PRO	LEU
4	TYR	THR	SER	TYR	THR	TYR	GLN	ALA	THR	PRO
5	MET	ASP	GLY	THR	LEU	LYS	THR	MET	LEU	GLU
6	ARG	TRP	ALA	ALA	ASP	SER	ASN	MET	GLN	LEU
7	SER	TYR	ASN	LEU	PRO	SER	ASP	TYR	THR	LEU
8	ILE	GLY	PRO	VAL	SER	ALA	ILE	SER	THR	THR
9	SER	VAL	GLN	GLN	ALA	ALA	THR	GLU	LEU	SER
10	ALA	VAL	TYR	ALA	ALA	GLN	GLY	VAL	SER	VAL
11	SER	VAL	SER	ALA	ASN	LYS	LEU	LEU	VAL	GLN
12	PRO	VAL	PRO	VAL	SER	SER	GLY	ALA	LYS	LEU

1	GLY	SER	HIS	MET	THR	LYS	PRO	ALA	PRO	ASP
2	PHE	GLY	GLY	ARG	TRP	LYS	HIS	VAL	ASN	HIS
3	PHE	ASP	GLU	ALA	PRO	THR	GLU	ILE	PRO	LEU
4	TYR	THR	SER	TYR	THR	TYR	GLN	ALA	THR	PRO
5	MET	ASP	GLY	THR	LEU	LYS	THR	MET	LEU	GLU
6	ARG	TRP	ALA	ALA	ASP	SER	ASN	MET	GLN	LEU
7	SER	TYR	ASN	LEU	PRO	SER	ASP	TYR	THR	LEU
8	ILE	GLY	PRO	VAL	SER	ALA	ILE	SER	THR	THR
9	SER	VAL	GLN	GLN	ALA	ALA	THR	GLU	LEU	SER
10	ALA	VAL	TYR	ALA	ALA	GLN	GLY	VAL	SER	VAL
11	SER	VAL	SER	ALA	ASN	LYS	LEU	LEU	VAL	GLN
12	PRO	VAL	PRO	VAL	SER	SER	GLY	ALA	LYS	LEU

1	GLY	SER	HIS	MET	THR	LYS	PRO	ALA	PRO	ASP
2	PHE	GLY	GLY	ARG	TRP	LYS	HIS	VAL	ASN	HIS
3	PHE	ASP	GLU	ALA	PRO	THR	GLU	ILE	PRO	LEU
4	TYR	THR	SER	TYR	THR	TYR	GLN	ALA	THR	PRO
5	MET	ASP	GLY	THR	LEU	LYS	THR	MET	LEU	GLU
6	ARG	TRP	ALA	ALA	ASP	SER	ASN	MET	GLN	LEU
7	SER	TYR	ASN	LEU	PRO	SER	ASP	TYR	THR	LEU
8	ILE	GLY	PRO	VAL	SER	ALA	ILE	SER	THR	THR
9	SER	VAL	GLN	GLN	ALA	ALA	THR	GLU	LEU	SER
10	ALA	VAL	TYR	ALA	ALA	GLN	GLY	VAL	SER	VAL
11	SER	VAL	SER	ALA	ASN	LYS	LEU	LEU	VAL	GLN
12	PRO	VAL	PRO	VAL	SER	SER	GLY	ALA	LYS	LEU