BMRB Entry 17250

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17250

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Title:
GNA1946
Deposition date:
2010-10-12
Original release date:
2012-07-25
Authors:
Bonvin, Alexandre; Neumoin, Alexey
Citation:

Citation: Neumoin, Alexey; Leonchiks, Ainars; Petit, Pierre; Vuillard, Laurent; Pizza, M.; Soriani, Marco; Boelens, Rolf; Bonvin, Alexandre. "1H, 13C and 15N assignment of the GNA1946 outer membrane lipoprotein from Neisseria meningitidis"  Biomol. NMR Assignments 5, 135-138 (2011).
PubMed: 21188561

Assembly members:

Assembly members:
GNA1946, polymer, 287 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Neisseria meningitidis   Taxonomy ID: 487   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GNA1946: MKTFFKTLSAAALALILAAC GGQKDSAPAASASAAADNGA AKKEIVFGTTVGDFGDMVKE QIQAELEKKGYTVKLVEFTD YVRPNLALAEGELDINVFQH KPYLDDFKKEHNLDITEVFQ VPTAPLGLYPGKLKSLEEVK DGSTVSAPNDPSNFARVLVM LDELGWIKLKDGINPLTASK ADIAENLKNIKIVELEAAQL PRSRADVDFAVVNGNYAISS GMKLTEALFQEPSFAYVNWS AVKTADKDSQWLKDVTEAYN SDAFKAYAHKRFEGYKSPAA WNEGAAK

Data sets:
Data typeCount
13C chemical shifts1186
15N chemical shifts282
1H chemical shifts1927

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GNA19461

Entities:

Entity 1, GNA1946 287 residues - Formula weight is not available

1   METLYSTHRPHEPHELYSTHRLEUSERALA
2   ALAALALEUALALEUILELEUALAALACYS
3   GLYGLYGLNLYSASPSERALAPROALAALA
4   SERALASERALAALAALAASPASNGLYALA
5   ALALYSLYSGLUILEVALPHEGLYTHRTHR
6   VALGLYASPPHEGLYASPMETVALLYSGLU
7   GLNILEGLNALAGLULEUGLULYSLYSGLY
8   TYRTHRVALLYSLEUVALGLUPHETHRASP
9   TYRVALARGPROASNLEUALALEUALAGLU
10   GLYGLULEUASPILEASNVALPHEGLNHIS
11   LYSPROTYRLEUASPASPPHELYSLYSGLU
12   HISASNLEUASPILETHRGLUVALPHEGLN
13   VALPROTHRALAPROLEUGLYLEUTYRPRO
14   GLYLYSLEULYSSERLEUGLUGLUVALLYS
15   ASPGLYSERTHRVALSERALAPROASNASP
16   PROSERASNPHEALAARGVALLEUVALMET
17   LEUASPGLULEUGLYTRPILELYSLEULYS
18   ASPGLYILEASNPROLEUTHRALASERLYS
19   ALAASPILEALAGLUASNLEULYSASNILE
20   LYSILEVALGLULEUGLUALAALAGLNLEU
21   PROARGSERARGALAASPVALASPPHEALA
22   VALVALASNGLYASNTYRALAILESERSER
23   GLYMETLYSLEUTHRGLUALALEUPHEGLN
24   GLUPROSERPHEALATYRVALASNTRPSER
25   ALAVALLYSTHRALAASPLYSASPSERGLN
26   TRPLEULYSASPVALTHRGLUALATYRASN
27   SERASPALAPHELYSALATYRALAHISLYS
28   ARGPHEGLUGLYTYRLYSSERPROALAALA
29   TRPASNGLUGLYALAALALYS

Samples:

sample: GNA1946, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 200 mM; H2O 95%; D2O 5%

sample_conditions: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsampleisotropicsample_conditions
2D 1H-13C HSQCsampleisotropicsample_conditions
3D HNCOsampleisotropicsample_conditions
3D HNCAsampleisotropicsample_conditions
3D HN(CO)CAsampleisotropicsample_conditions
3D HN(CA)COsampleisotropicsample_conditions
3D HCCH-TOCSYsampleisotropicsample_conditions
3D CBCA(CO)NHsampleisotropicsample_conditions
3D 1H-15N NOESYsampleisotropicsample_conditions
3D 1H-13C NOESYsampleisotropicsample_conditions

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
EMBL CAM07784 CAM11078 CAX50901 CBA03352 CBA03733
GB AAF42275 AAF42629 AAF42630 AAF42631 AAF42632
REF NP_274940 WP_002214810 WP_002218060 WP_002221629 WP_002223094

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks