BMRB Entry 17241

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PDB ID: 2l4j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17241
MolProbity Validation Chart

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Title:
YAP WW2
Deposition date:
2010-10-07
Original release date:
2011-03-30
Authors:
Webb, Claire; Upadhyay, Abhishek; Furutani-Seiki, Makoto; Bagby, Stefan
Citation:

Citation: Webb, Claire; Upadhyay, Abhishek; Giuntini, Francesca; Eggleston, Ian; Furutani-Seiki, Makoto; Ishima, Rieko; Bagby, Stefan. "Structural Features and Ligand Binding Properties of Tandem WW Domains from YAP and TAZ, Nuclear Effectors of the Hippo Pathway."  Biochemistry 50, 3300-3309 (2011).
PubMed: 21417403

Assembly members:

Assembly members:
entity, polymer, 46 residues, 5130.625 Da.

Natural source:

Natural source:   Common Name: Japanese medaka   Taxonomy ID: 8090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryzias latipes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSV281

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSPNSSPASGPLPEGWEQAI TPEGEIYYINHKNKTTSWLD PRLETR

Data sets:
Data typeCount
13C chemical shifts193
15N chemical shifts46
1H chemical shifts264

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1YAP1

Entities:

Entity 1, YAP 46 residues - 5130.625 Da.

The first 5 residues (residues 177-181) are remains of a non-native affinity tag.

1   GLYSERPROASNSERSERPROALASERGLY
2   PROLEUPROGLUGLYTRPGLUGLNALAILE
3   THRPROGLUGLYGLUILETYRTYRILEASN
4   HISLYSASNLYSTHRTHRSERTRPLEUASP
5   PROARGLEUGLUTHRARG

Related Database Links:

PDB 2L4J

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