BMRB Entry 17216
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17216
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Citation: Bourbigot, Sarah; Ramalanjaona, Nick; Boudier, Christian; Salgado, Gilmar; Roques, Bernard; Mely, Yves; Bouaziz, Serge; Morellet, Nelly. "How the HIV-1 Nucleocapsid Protein Binds and Destabilises the (-)Primer Binding Site During Reverse Transcription" J. Mol. Biol. 383, 1112-1128 (2008).
PubMed: 18773912
Assembly members:
PPBS, polymer, 12 residues, Formula weight is not available
NCp7(12-55), polymer, 44 residues, Formula weight is not available
Natural source: Common Name: Human immunodeficiency virus 1 Taxonomy ID: 11676 Superkingdom: not available Kingdom: not available Genus/species: Lentivirus not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
PPBS: GCCCTGTTGGGC
NCp7(12-55): NVKCFNCGKEGHTARNCRAP
RKKGCWKCGKEGHQMKDCTE
RQAN
- chemical_rates
| Data type | Count |
| kinetic rates | 4 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PPBS | 1 |
| 2 | NCp7(12-55) | 2 |
Entities:
Entity 1, PPBS 12 residues - Formula weight is not available
| 1 | DG | DC | DC | DC | DT | DG | DT | DT | DG | DG | ||||
| 2 | DG | DC |
Entity 2, NCp7(12-55) 44 residues - Formula weight is not available
| 1 | ASN | VAL | LYS | CYS | PHE | ASN | CYS | GLY | LYS | GLU | ||||
| 2 | GLY | HIS | THR | ALA | ARG | ASN | CYS | ARG | ALA | PRO | ||||
| 3 | ARG | LYS | LYS | GLY | CYS | TRP | LYS | CYS | GLY | LYS | ||||
| 4 | GLU | GLY | HIS | GLN | MET | LYS | ASP | CYS | THR | GLU | ||||
| 5 | ARG | GLN | ALA | ASN |
Samples:
sample_1: PPBS 1 mM; NCp7(12-55) 1 mM; D2O 100%
sample_2: PPBS 1 mM; NCp7(12-55) 1 mM; D2O 10%; H2O 90%
sample_conditions_1: pH: 6.5; pressure: ambient atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D EXSY | sample_1 | isotropic | sample_conditions_1 |
| 2D EXSY | sample_2 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - processing
FELIX, Accelrys Software Inc. - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 2217 25532 |
| PDB | |
| DBJ | BAA12988 BAA12996 BAB85751 BAB85752 BAC02530 |
| EMBL | CAB58976 CAB58988 CAB98186 CAP69989 CAP69993 |
| GB | AAA44201 AAA45076 AAA76686 AAB05598 AAB21888 |
| PIR | FOVWLV |
| PRF | 1102247B 1103299C |
| REF | NP_057849 NP_057850 NP_579881 |
| SP | P03347 P03348 P03366 P03367 P04585 |
| AlphaFold | P03347 P03348 P03366 P03367 P04585 |