BMRB Entry 17170

Name: Resonance assignments of the Myb2 DNA binding domain in complex with its promoter MRE-1 from Trichomonas vaginalis
Published: 2012-08-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17170

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of the Myb2 DNA binding domain in complex with its promoter MRE-1 from Trichomonas vaginalis

Deposition date:

2010-09-07

Original release date:

2012-08-01

Authors:

Huang, Tai-Huang; Jiang, Ingjye; Tsai, Chen-Kun; Naik, Mandar; Chang, Chi-Fon; Wu, Wen-Jin

Citation:

Citation: Huang, Tai-huang; Jiang, Ingjye; Tsai, Chen-Kun. "Resonance assignments of the Myb2 DNA binding domain in complex with its promoter MRE-1 from Trichomonas vaginalis" .

Assembly members:

Assembly members:
tvMyb2_MRE-1, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Trichomonas vaginalis Taxonomy ID: 5722 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trichomonas vaginalis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tvMyb2_MRE-1: MVQVNLKAAKKQKFTPEEDE MLKRAVAQHGSDWKMIAATF PNRNARQCRDRWKNYLAPSI SHTPWTAEEDALLVQKIQEY GRQWAIIAKFFPGRTDIHIK NRWVTISNKLGIPQTQQMLE HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	476
15N chemical shifts	112
1H chemical shifts	723

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tvMyb2	1

Entities:

Entity 1, tvMyb2 126 residues - Formula weight is not available

1

MET

VAL

GLN

VAL

ASN

LEU

LYS

ALA

LYS

2

LYS

GLN

LYS

PHE

THR

PRO

GLU

ASP

GLU

3

MET

LEU

LYS

ARG

ALA

VAL

ALA

GLN

HIS

GLY

4

SER

ASP

TRP

LYS

MET

ILE

ALA

THR

PHE

5

PRO

ASN

ARG

ASN

ALA

ARG

GLN

CYS

ARG

ASP

6

ARG

TRP

LYS

ASN

TYR

LEU

ALA

PRO

SER

ILE

7

SER

HIS

THR

PRO

TRP

THR

ALA

GLU

ASP

8

ALA

LEU

VAL

GLN

LYS

ILE

GLN

GLU

TYR

9

GLY

ARG

GLN

TRP

ALA

ILE

ALA

LYS

PHE

10

PHE

PRO

GLY

ARG

THR

ASP

ILE

HIS

ILE

LYS

11

ASN

ARG

TRP

VAL

THR

ILE

SER

ASN

LYS

LEU

12

GLY

ILE

PRO

GLN

THR

GLN

MET

LEU

GLU

13

HIS

Samples:

sample_1: EDTA 1 mM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; H2O 93%; D2O 7%; tvMyb2_MRE-1, [U-100% 13C; U-100% 15N], 0.6 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz

PDB	3OSF 3OSG
GB	AAX51242 EAY06671
REF	XP_001318894