BMRB Entry 17161

Assembly:

Entities:

Entity 1, Rna14p 53 residues - Formula weight is not available

Start methionine derives from the cloning site within the expression plasmid

Entity 2, Rna15p 109 residues - Formula weight is not available

The Gly-His-Met sequence derives from the cloning vector and is present at the N-terminus of the peptide following cleavage and removal of the His-tag by TEV protease

Samples:

sample_1: Rna14p, [U-100% 13C; U-100% 15N; U-70% 2H], 1.0 mM; Rna15p, [U-100% 13C; U-100% 15N; U-70% 2H], 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_2: Rna14p, [U-99% 15N], 0.2-1.0 mM; Rna15p, [U-99% 15N], 0.2-1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_3: Rna14p, [U-99% 15N], 0.2 mM; Rna15p, [U-99% 15N], 0.2 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_4: Rna14p, [U-99% 13C; U-99% 15N], 0.4 mM; Rna15p, [U-99% 13C; U-99% 15N], 0.4 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_5: Rna14p, [U-99% 13C; U-99% 15N], 0.4 mM; Rna15p, [U-99% 13C; U-99% 15N], 0.4 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_6: Rna14p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; Rna15p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_7: Rna14p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; Rna15p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_8: Rna14p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; Rna15p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_9: Rna14p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; Rna15p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_10: Rna14p, [U-10% 13C; U-99% 15N], 1.0 mM; Rna15p, [U-10% 13C; U-99% 15N], 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Software:

TOPSPIN v2.0, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

• Bruker Avance 700 MHz
• Bruker Avance 800 MHz