BMRB Entry 17157

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PDB ID: 2l30
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17157
MolProbity Validation Chart

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Title:
DNA repair protein zinc finger 1
Deposition date:
2010-08-30
Original release date:
2011-01-27
Authors:
Neuhaus, David; Eustermann, Sebastian; Yang, Ji-Chun; Videler, Hortense
Citation:

Citation: Eustermann, Sebastian; Videler, Hortense; Yang, Ji-Chun; Cole, Paul; Gruszka, Dominika; Veprintsev, Dmitry; Neuhaus, David. "The DNA-Binding Domain of Human PARP-1 Interacts with DNA Single-Strand Breaks as a Monomer through Its Second Zinc Finger."  J. Mol. Biol. 407, 149-170 (2011).
PubMed: 21262234

Assembly members:

Assembly members:
PARP-1_Finger_1, polymer, 108 residues, 12132.885 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PARP-1_Finger_1: MAESSDKLYRVEYAKSGRAS CKKCSESIPKDSLRMAIMVQ SPMFDGKVPHWYHFSCFWKV GHSIRHPDVEVDGFSELRWD DQQKVKKTAEAGGVTGKGQD GIGSKAEK

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts102
1H chemical shifts703

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PARP-1 Finger 11
2ZINC ION2

Entities:

Entity 1, PARP-1 Finger 1 108 residues - 12132.885 Da.

1   METALAGLUSERSERASPLYSLEUTYRARG
2   VALGLUTYRALALYSSERGLYARGALASER
3   CYSLYSLYSCYSSERGLUSERILEPROLYS
4   ASPSERLEUARGMETALAILEMETVALGLN
5   SERPROMETPHEASPGLYLYSVALPROHIS
6   TRPTYRHISPHESERCYSPHETRPLYSVAL
7   GLYHISSERILEARGHISPROASPVALGLU
8   VALASPGLYPHESERGLULEUARGTRPASP
9   ASPGLNGLNLYSVALLYSLYSTHRALAGLU
10   ALAGLYGLYVALTHRGLYLYSGLYGLNASP
11   GLYILEGLYSERLYSALAGLULYS

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2DMJ 2L30 3ODA 4DQY 4OPX 4OQA 4OQB
EMBL CAA39606
REF XP_011359180

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