BMRB Entry 17139

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17139

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Title:
Structure of ARC92VBD/MED25ACID
Deposition date:
2010-08-21
Original release date:
2011-06-07
Authors:
Milbradt, Alexander; Sun, Zhen-Yu; Selenko, Philipp; Takeuchi, Koh; Naar, Anders; Wagner, Gerhard
Citation:

Citation: Milbradt, Alexander; Kulkarni, Madhura; Yi, Tingfang; Takeuchi, Koh; Sun, Zhen-Yu; Luna, Rafael; Selenko, Philipp; Naar, Anders; Wagner, Gerhard. "Structure of the VP16 transactivator target in the Mediator"  Nat. Struct. Mol. Biol. 18, 410-415 (2011).
PubMed: 21378963

Assembly members:

Assembly members:
ARC92VBD, polymer, 166 residues, 37940.359 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p-his-parallel

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ARC92VBD: GEFGQQSVSNKLLAWSGVLE WQEKPKPASVDANTKLTRSL PCQVYVNHGENLKTEQWPQK LIMQLIPQQLLTTLGPLFRN SRMVQFHFTNKDLESLKGLY RIMGNGFAGCVHFPHTAPCE VRVLMLLYSSKKKIFMGLIP YDQSGFVNGIRQVITNHKQV QQQKLE

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts156
1H chemical shifts1052

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ARC92VBD1

Entities:

Entity 1, ARC92VBD 166 residues - 37940.359 Da.

Residues 388-390 represent a cloning site and are not part of the natural sequence

1   GLYGLUPHEGLYGLNGLNSERVALSERASN
2   LYSLEULEUALATRPSERGLYVALLEUGLU
3   TRPGLNGLULYSPROLYSPROALASERVAL
4   ASPALAASNTHRLYSLEUTHRARGSERLEU
5   PROCYSGLNVALTYRVALASNHISGLYGLU
6   ASNLEULYSTHRGLUGLNTRPPROGLNLYS
7   LEUILEMETGLNLEUILEPROGLNGLNLEU
8   LEUTHRTHRLEUGLYPROLEUPHEARGASN
9   SERARGMETVALGLNPHEHISPHETHRASN
10   LYSASPLEUGLUSERLEULYSGLYLEUTYR
11   ARGILEMETGLYASNGLYPHEALAGLYCYS
12   VALHISPHEPROHISTHRALAPROCYSGLU
13   VALARGVALLEUMETLEULEUTYRSERSER
14   LYSLYSLYSILEPHEMETGLYLEUILEPRO
15   TYRASPGLNSERGLYPHEVALASNGLYILE
16   ARGGLNVALILETHRASNHISLYSGLNVAL
17   GLNGLNGLNLYSLEUGLU

Related Database Links:

BMRB 17323
PDB 2KY6 2L23 2L6U 2XNF
DBJ BAE29135 BAF82149 BAG51583 BAG61008
EMBL CAB66680 CAE84581
GB AAG15589 AAH21333 AAH24312 AAH31138 AAH65297
REF NP_001075914 NP_001163897 NP_083641 NP_112235 XP_001115473
SP A2VE44 Q71SY5 Q8VCB2
TPG DAA19611
AlphaFold A2VE44 Q71SY5 Q8VCB2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks