BMRB Entry 17133

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17133

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Title:
Chemical shift assignments for the RanBP2-type ZnF from Ewing's Sarcoma (EWS)
Deposition date:
2010-08-18
Original release date:
2011-01-25
Authors:
Leung, Wilfred; Mansfield, Robyn
Citation:

Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA."  J. Mol. Biol. 407, 273-283 (2011).
PubMed: 21256132

Assembly members:

Assembly members:
EWS, polymer, 39 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EWS: GSNVQHRAGDWQCPNPGCGN QNFAWRTECNQCKAPKPEG

Data sets:
Data typeCount
13C chemical shifts120
15N chemical shifts38
1H chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EWS1
2ZN2

Entities:

Entity 1, EWS 39 residues - Formula weight is not available

1   GLYSERASNVALGLNHISARGALAGLYASP
2   TRPGLNCYSPROASNPROGLYCYSGLYASN
3   GLNASNPHEALATRPARGTHRGLUCYSASN
4   GLNCYSLYSALAPROLYSPROGLUGLY

Entity 2, ZN - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: EWS, [U-100% 13C; U-100% 15N], 0.5 mM; D2O 5%; DSS 20 uM; sodium chloride 100 mM; sodium phosphate 25 mM; DTT 1 mM; H2O 95%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

DBJ BAB29301 BAB31732 BAB71145 BAB71252 BAE02261
EMBL CAA47350 CAA51489 CAA55815 CAA69177 CAG30376
GB AAH04817 AAH11048 AAH63928 AAH68226 AAH72442
PRF 1818357A
REF NP_001020803 NP_001089902 NP_001103270 NP_001156757 NP_001156758
SP Q01844 Q61545
TPG DAA20531
AlphaFold Q01844 Q61545

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks