BMRB Entry 17131

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17131

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Title:
NMR resonance assignment of the N-terminal domain of Latrodectus hesperus (black widow) major ampullate spider silk fibroin 1
Deposition date:
2010-08-16
Original release date:
2010-11-10
Authors:
Kessler, Horst; Hagn, Franz
Citation:

Citation: Hagn, Franz; Thamm, Christopher; Scheibel, Thomas; Kessler, Horst. "pH-dependent dimerization and salt-dependent stabilization of the N-terminal domain of spider dragline silk--implications for fiber formation."  Angew. Chem. Int. Ed. Engl. 50, 310-313 (2011).

Assembly members:

Assembly members:
N1, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: black widow spider   Taxonomy ID: 256737   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Latrodectus hesperus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N1: MGQANTPWSSKANADAFINS FISAASNTGSFSQDQMEDMS LIGNTLMAAMDNMGGRITPS KLQALDMAFASSVAEIAASE GGDLGVTTNAIADALTSAFY QTTGVVNSRFISEIRSLIGM FAQASANDVYAS

Data sets:
Data typeCount
13C chemical shifts458
15N chemical shifts131
1H chemical shifts496

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N11

Entities:

Entity 1, N1 132 residues - Formula weight is not available

1   METGLYGLNALAASNTHRPROTRPSERSER
2   LYSALAASNALAASPALAPHEILEASNSER
3   PHEILESERALAALASERASNTHRGLYSER
4   PHESERGLNASPGLNMETGLUASPMETSER
5   LEUILEGLYASNTHRLEUMETALAALAMET
6   ASPASNMETGLYGLYARGILETHRPROSER
7   LYSLEUGLNALALEUASPMETALAPHEALA
8   SERSERVALALAGLUILEALAALASERGLU
9   GLYGLYASPLEUGLYVALTHRTHRASNALA
10   ILEALAASPALALEUTHRSERALAPHETYR
11   GLNTHRTHRGLYVALVALASNSERARGPHE
12   ILESERGLUILEARGSERLEUILEGLYMET
13   PHEALAGLNALASERALAASNASPVALTYR
14   ALASER

Related Database Links:

GB ABR68856 ABR68857 ABY67402 ABY67406 ABY67410

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks