BMRB Entry 17079

Click here to enlarge.

PDB ID: 2l1b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17079
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution NMR structure ov the chromobox protein Cbx7 with H3K27me3
Deposition date:
2010-07-27
Original release date:
2010-08-18
Authors:
Kaustov, Lilia; Lemak, Alexander; Fares, Christophe; Gutmanas, Aleksandras; Muhandiram, Ranjith; Quang, Hui; Loppnau, Peter; Min, Jinrong; Edwards, Al; Arrowsmith, Cheryl
Citation:

Citation: Kaustov, Lilia; Ouyang, Hui; Amaya, Maria; Lemak, Alexander; Nady, Nataliya; Duan, Shili; Wasney, Gregory; Li, Zhihong; Vedadi, Masoud; Schapira, Matthieu; Min, Jinrong; Arrowsmith, Cheryl. "Recognition and specificity determinants of the human cbx chromodomains."  J. Biol. Chem. 286, 521-529 (2011).
PubMed: 21047797

Assembly members:

Assembly members:
Cbx7, polymer, 56 residues, 6816.929 Da.
H3K27me3, polymer, 15 residues, 1515.792 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a-mhl

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cbx7: GEQVFAVESIRKKRVRKGKV EYLVKWKGWPPKYSTWEPEE HILDPRLVMAYEEKEE
H3K27me3: QLATKAARXSAPATG

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts50
1H chemical shifts473

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cbx71
2H3K27me32

Entities:

Entity 1, Cbx7 56 residues - 6816.929 Da.

1   GLYGLUGLNVALPHEALAVALGLUSERILE
2   ARGLYSLYSARGVALARGLYSGLYLYSVAL
3   GLUTYRLEUVALLYSTRPLYSGLYTRPPRO
4   PROLYSTYRSERTHRTRPGLUPROGLUGLU
5   HISILELEUASPPROARGLEUVALMETALA
6   TYRGLUGLULYSGLUGLU

Entity 2, H3K27me3 15 residues - 1515.792 Da.

1   GLNLEUALATHRLYSALAALAARGM3LSER
2   ALAPROALATHRGLY

Related Database Links:

BMRB 15674 16778 17072
PDB 2K1B 2KVM 2L12 2L1B 4MN3 4X3K 4X3S 4X3T 4X3U
DBJ BAI46775
GB AAH21398 AAH51773 AAH62392 AAR26721 AAX94038
REF NP_001179547 NP_001247702 NP_659060 NP_783640 NP_954548
SP O95931 P60889 Q8VDS3
TPG DAA29144
AlphaFold O95931 P60889 Q8VDS3 Q92133

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks