BMRB Entry 17050

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PDB ID: 2xk0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17050
MolProbity Validation Chart

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Title:
Tudor domain from Drosophila Polycomblike
Deposition date:
2010-07-08
Original release date:
2010-08-12
Authors:
Friberg, A.; Oddone, A.; Klymenko, T.; Mueller, J.; Sattler, M.
Citation:

Citation: Friberg, Anders; Oddone, Anna; Klymenko, Tetyana; Muller, Jurg; Sattler, Michael. "Structure of an atypical Tudor domain in the Drosophila Polycomblike protein."  Protein Sci. 19, 1906-1916 (2010).
PubMed: 20669242

Assembly members:

Assembly members:
POLYCOMB_PROTEIN_PCL, polymer, 69 residues, 7578.4194 Da.

Natural source:

Natural source:   Common Name: FRUIT FLY   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-24d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
POLYCOMB_PROTEIN_PCL: GAMAPPVAAPSPAVTYALQE DVFIKCNDGRFYLGTIIDQT SDQYLIRFDDQSEQWCEPDK LRKLGGGSS

Data sets:
Data typeCount
13C chemical shifts220
15N chemical shifts66
1H chemical shifts442

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1POLYCOMB PROTEIN PCL1

Entities:

Entity 1, POLYCOMB PROTEIN PCL 69 residues - 7578.4194 Da.

Three first residues is a cloning artifact.

1   GLYALAMETALAPROPROVALALAALAPRO
2   SERPROALAVALTHRTYRALALEUGLNGLU
3   ASPVALPHEILELYSCYSASNASPGLYARG
4   PHETYRLEUGLYTHRILEILEASPGLNTHR
5   SERASPGLNTYRLEUILEARGPHEASPASP
6   GLNSERGLUGLNTRPCYSGLUPROASPLYS
7   LEUARGLYSLEUGLYGLYGLYSERSER

Related Database Links:

UNP PCL_DROME
PDB 2XK0
GB AAA64457 AAF57748 AAL68389 ACL83738 ACL88860
REF NP_001261067 NP_476672 XP_001974621 XP_002034393 XP_002082031
SP Q24459
AlphaFold Q9V8C2 Q24459

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CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks