BMRB Entry 17030

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PDB ID: 2l05
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17030
MolProbity Validation Chart

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Title:
Solution NMR Structure of the Ras-binding domain of Serine/threonine-protein kinase B-raf from Homo sapiens, Northeast Structural Genomics Consortium Target HR4694F
Deposition date:
2010-06-30
Original release date:
2010-07-07
Authors:
Aramini, James; Janjua, Haleema; Ciccosanti, Colleen; Shastry, Ritu; Huang, Yuanpeng; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano
Citation:

Citation: Aramini, James; Janjua, Haleema; Ciccosanti, Colleen; Shastry, Ritu; Huang, Yuanpeng; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano. "Solution NMR Structure of the Ras-binding domain of Serine/threonine-protein kinase B-raf from Homo sapiens, Northeast Structural Genomics Consortium Target HR4694F"  To be published ., .-..

Assembly members:

Assembly members:
HR4694F, polymer, 95 residues, 9778.635 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 14-15C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR4694F: MGHHHHHHSHMPKSPQKPIV RVFLPNKQRTVVPARCGVTV RDSLKKALMMRGLIPECCAV YRIQDGEKKPIGWDTDISWL TGEELHVEVLENVPL

Data typeCount
13C chemical shifts394
15N chemical shifts89
1H chemical shifts640

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1HR4694F1

Entities:

Entity 1, HR4694F 95 residues - 9778.635 Da.

N-terminal MGHHHHHHSHM tag.

1   METGLYHISHISHISHISHISHISSERHIS
2   METPROLYSSERPROGLNLYSPROILEVAL
3   ARGVALPHELEUPROASNLYSGLNARGTHR
4   VALVALPROALAARGCYSGLYVALTHRVAL
5   ARGASPSERLEULYSLYSALALEUMETMET
6   ARGGLYLEUILEPROGLUCYSCYSALAVAL
7   TYRARGILEGLNASPGLYGLULYSLYSPRO
8   ILEGLYTRPASPTHRASPILESERTRPLEU
9   THRGLYGLUGLULEUHISVALGLUVALLEU
10   GLUASNVALPROLEU

Related Database Links:

PDB 2L05
DBJ BAD19009 BAE02474 BAE24384 BAI47380
EMBL CAA46301 CAA47436 CAB81555
GB AAA35609 AAA49492 AAA49493 AAI01758 AAI12080
PIR I51153
REF NP_004324 NP_647455 NP_990633 XP_001070228 XP_001496314
SP P15056 P28028 P34908 Q04982
TPG DAA30146
AlphaFold P15056 P28028 P34908 Q04982

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