BMRB Entry 17008

Assembly:

Entities:

Entity 1, SR10 151 residues - 17145.258 Da.

The construct studied has LEHHHHHH purification tag on C-terminus.

Samples:

SR10.026: SR10, [U-100% 13C; U-100% 15N; U-100% 2H], 0.8 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM; sodium chloride 100 mM; protease inhibitor 1 x; MES 20 mM; DSS 50 uM; H2O 90%; D2O 10%

SR10.022: SR10, [U-5% 13C; U-100% 15N], 1.2 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM; sodium chloride 100 mM; protease inhibitor 1 x; MES 20 mM; DSS 50 uM; H2O 90%; D2O 10%

SR10.022_2: SR10, [U-5% 13C; U-100% 15N], 1.2 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM; sodium chloride 200 mM; protease inhibitor 1 x; MES 20 mM; DSS 50 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinemen, structure solution

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - geometry optimization, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - data analysis, peak picking

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

PALES, PALES (Zweckstetter, Bax) - geometry optimization

NMR spectrometers:

• Bruker Avance 800 MHz
• Varian INOVA 600 MHz