BMRB Entry 16981

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16981
MolProbity Validation Chart

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Title:
NMR Structure of the SARS Coronavirus Nonstructural Protein Nsp7 in Solution at pH 6.5
Deposition date:
2010-06-07
Original release date:
2010-07-08
Authors:
Johnson, Margaret; Jaudzems, Kristaps; Wuthrich, Kurt
Citation:

Citation: Johnson, Margaret; Jaudzems, Kristaps; Wuthrich, Kurt. "NMR Structure of the SARS-CoV Nonstructural Protein 7 in Solution at pH6.5."  J. Mol. Biol. 402, 619-628 (2010).
PubMed: 20709084

Assembly members:

Assembly members:
nsp7, polymer, 85 residues, 9472.080 Da.

Natural source:

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: virus   Kingdom: not available   Genus/species: Betacoronavirus Severe acute respiratory syndrome-related coronavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nsp7: GHSKMSDVKCTSVVLLSVLQ QLRVESSSKLWAQCVQLHND ILLAKDTTEAFEKMVSLLSV LLSMQGAVDINRLCEEMLDN RATLQ

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts93
1H chemical shifts630

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp71

Entities:

Entity 1, nsp7 85 residues - 9472.080 Da.

1   GLYHISSERLYSMETSERASPVALLYSCYS
2   THRSERVALVALLEULEUSERVALLEUGLN
3   GLNLEUARGVALGLUSERSERSERLYSLEU
4   TRPALAGLNCYSVALGLNLEUHISASNASP
5   ILELEULEUALALYSASPTHRTHRGLUALA
6   PHEGLULYSMETVALSERLEULEUSERVAL
7   LEULEUSERMETGLNGLYALAVALASPILE
8   ASNARGLEUCYSGLUGLUMETLEUASPASN
9   ARGALATHRLEUGLN

Samples:

sample_1: nsp7, [U-98% 13C; U-98% 15N], 2 mM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 10 mM; sodium azide 3 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.253 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-resolved [1H,1H]-NOESYsample_1isotropicsample_conditions_1
3D 13C-resolved [1H,1H]-NOESY (aliphatic carbons)sample_1isotropicsample_conditions_1
3D 13C-resolved [1H,1H]-NOESY (aromatic carbons)sample_1isotropicsample_conditions_1
2D [15N,1H]-HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HC(C)H-TOCSYsample_1isotropicsample_conditions_1
2D [13C,1H]-HSQCsample_1isotropicsample_conditions_1

Software:

UNIO v1.0.4, Torsten Herrmann - structure solution

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

ATNOS v2.2, Torsten Herrmann, Peter G ntert, Kurt Wuthrich - structure solution

CANDID v2.2, Torsten Herrmann, Peter G ntert, Kurt Wuthrich - structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

TOPSPIN, Bruker Biospin - collection, data analysis

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF NP_828849 NP_828850 NP_828865
SP P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9
AlphaFold P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks