BMRB Entry 16957

Name: Solution structure of the voltage-sensing domain of KvAP
Published: 2010-09-24

Click here to enlarge.

PDB ID: 2kyh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16957
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the voltage-sensing domain of KvAP

Deposition date:

2010-05-26

Original release date:

2010-09-24

Authors:

Butterwick, Joel; MacKinnon, Roderick

Citation:

Citation: Butterwick, Joel; MacKinnon, Roderick. "Solution structure and phospholipid interactions of the isolated voltage-sensor domain from KvAP." J. Mol. Biol. 403, 591-606 (2010).
PubMed: 20851706

Assembly members:

Assembly members:
KvAP, polymer, 147 residues, 16197.432 Da.

Natural source:

Natural source: Common Name: Aeropyrum pernix Taxonomy ID: 56636 Superkingdom: Archaea Kingdom: not available Genus/species: Aeropyrum pernix

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE60

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KvAP: LRGLSDLGGRVRNIGDVMEH PLVELGVSYAALLSVIVVVV EYTMQLSGEYLVRLYLVDLI LVIILWADYAYRAYKSGDPA GYVKKTLYEIPALVPAGLLA LIEGHLAGLGLFRLVRLLRF LRILLIISRGSKFLSAIADA ADKLVPR

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	353
15N chemical shifts	231
1H chemical shifts	233

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	voltage-sensor domain of KvAP	1

Entities:

Entity 1, voltage-sensor domain of KvAP 147 residues - 16197.432 Da.

1

LEU

ARG

GLY

LEU

SER

ASP

LEU

GLY

ARG

2

VAL

ARG

ASN

ILE

GLY

ASP

VAL

MET

GLU

HIS

3

PRO

LEU

VAL

GLU

LEU

GLY

VAL

SER

TYR

ALA

4

ALA

LEU

SER

VAL

ILE

VAL

5

GLU

TYR

THR

MET

GLN

LEU

SER

GLY

GLU

TYR

6

LEU

VAL

ARG

LEU

TYR

LEU

VAL

ASP

LEU

ILE

7

LEU

VAL

ILE

LEU

TRP

ALA

ASP

TYR

ALA

8

TYR

ARG

ALA

TYR

LYS

SER

GLY

ASP

PRO

ALA

9

GLY

TYR

VAL

LYS

THR

LEU

TYR

GLU

ILE

10

PRO

ALA

LEU

VAL

PRO

ALA

GLY

LEU

ALA

11

LEU

ILE

GLU

GLY

HIS

LEU

ALA

GLY

LEU

GLY

12

LEU

PHE

ARG

LEU

VAL

ARG

LEU

ARG

PHE

13

LEU

ARG

ILE

LEU

ILE

SER

ARG

GLY

14

SER

LYS

PHE

LEU

SER

ALA

ILE

ALA

ASP

ALA

15

ALA

ASP

LYS

LEU

VAL

PRO

ARG

Samples:

sample_N: KvAP, [U-98% 15N], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_Nd2o: KvAP, [U-98% 15N], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; D2O 99%

sample_DCN: KvAP, [U-100% 13C; U-100% 15N; U-50% 2H], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_CN: KvAP, [U-98% 13C; U-98% 15N], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; D2O 99%

sample_methyl: KvAP, [13CH3/12CD2]-Leu,Val;[13CH3]-Ile, 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_Arg: KvAP, [U-98% 13C; U-98% 15N]-Arg, 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_LGA: KvAP, [98% 1-13C,98% 15N]-Leu; [98% 2-13C]-Gly; [98% 2,3-13C]-Ala, 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_ILV: KvAP, [98% 15N]-Ile; [98% 2-13C]-Leu; [98% 1-13C]-Val, 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_10C: KvAP, [U-10% 13C], 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; D2O 99%

sample_conditions_1: ionic strength: 40 mM; pH: 7.0; pressure: 1 atm; temperature: 318 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_N	isotropic	sample_conditions_1
3D TROSY HNCO	sample_DCN	isotropic	sample_conditions_1
3D TROSY HNCA	sample_DCN	isotropic	sample_conditions_1
3D TROSY HN(CO)CA	sample_DCN	isotropic	sample_conditions_1
3D HCCH-COSY	sample_CN	isotropic	sample_conditions_1
3D TROSY HNCACB	sample_DCN	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_N	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_CN	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_methyl	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_DCN	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_Nd2o	isotropic	sample_conditions_1
2D HN(CO)	sample_LGA	isotropic	sample_conditions_1
2D H(N)CO	sample_LGA	isotropic	sample_conditions_1
2D HN(CA)	sample_LGA	isotropic	sample_conditions_1
2D H(N)CA	sample_LGA	isotropic	sample_conditions_1
2D HN(CO)	sample_ILV	isotropic	sample_conditions_1
2D H(N)CO	sample_ILV	isotropic	sample_conditions_1
2D HN(CA)	sample_ILV	isotropic	sample_conditions_1
2D H(N)CA	sample_ILV	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_LGA	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_ILV	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_Arg	isotropic	sample_conditions_1
2D HN(CA)	sample_Arg	isotropic	sample_conditions_1
2D H(N)CA	sample_Arg	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_DCN	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_Nd2o	isotropic	sample_conditions_1
3D 1H-13C NOESYaro	sample_CN	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_10C	isotropic	sample_conditions_1

PDB	1ORQ 1ORS 2A0L 2KYH
DBJ	BAA79939
SP	Q9YDF8
AlphaFold	Q9YDF8

BMRB Entry 16957

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: